Phosphoproteomic analysis of Bacillus subtilis
EMSL Project ID
48053
Abstract
Ser/Thr phosphorylation is a key mechanism of regulation in eukaryotic cells, but its role in prokaryotes is far less clear. In the past ~5 years, phosphoproteomic approaches have found that a large number of proteins (>75) in both Gram positive and Gram negative bacterial cells are phosphorylated on either a Ser or a Thr residue. Although the particular kinases responsible for these modifications are not known, Ser/Thr kinases have been described in phylogenetically diverse bacteria. They mediate processes including biofilm formation and the production of the bacterial cell wall. A key goal of microbiology is the identification of the substrates of these kinases in order to understand the regulation of these processes. Our proposed work will identify the substrates of a single Ser/Thr kinase in the bacterium Bacillus subtilis, and thereby offer new insight into the strategies used by this industrially important organism to regulate the production of secreted, extracellular proteins such as cellulose degrading enzymes. The automated IMAC technology developed by Dr. Paša-Tolic at EMSL will provide an essential and novel basis for our analysis. Thus, this work is central to the mission of the EMSL to provide solutions to the nation's energy production challenges such as those provided by biofuel production.
Project Details
Project type
Limited Scope
Start Date
2013-05-07
End Date
2013-07-07
Status
Closed
Released Data Link
Team
Principal Investigator