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Substrate Oxidation and Protein Synthesis Integration in a Complex Biological System


EMSL Project ID
48162

Abstract

These investigations will explore the complex interactions involved in cellular metabolism and protein synthesis in accordance with the EMSL Science Theme: Biological Interactions and Dynamics. We will use a complex model employing the pig neonatal heart and brain in vivo to link intra-cellular regulatory networks that control the response of cells to their environment. The neonatal heart in particular provides an excellent model for all cell systems as it undergoes rapid growth through cell proliferation and expansion and adapts to quickly to physiological changes. The neonatal brain is also undergoing rapid development but is extremely vulnerable to insults. These studies will have broad applications to protein synthesis and metabolomics extending past our models. Regulation of these pathways is relatively conserved through evolution. Therefore, the defined principles and techniques will be applicable to metabolomics and protein synthesis in microbes with specific EMSL interest.

Project Details

Start Date
2013-11-13
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Michael Portman
Institution
Seattle Children's Research Institute

Team Members

Aaron Olson
Institution
Seattle Children's Research Institute

Nancy Isern
Institution
Environmental Molecular Sciences Laboratory

Ljiljana Pasa-Tolic
Institution
Environmental Molecular Sciences Laboratory

Related Publications

Kajimoto M, CM O'Kelly-Priddy, DR Ledee, C Xu, NG Isern, A Olson, and MA Portman. 2014. "Effects of Continuous Triiodothyronine Infusion on Citric Acid Cycle in the Normal Immature Swine Heart under Extracorporeal Membrane Oxygenation in vivo." American Journal of Physiology. Heart and Circulatory Physiology. doi:10.1152/ajpheart.00964.2013