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The role of protein-mineral interactions for protein oxidation and hydrolysis


EMSL Project ID
48183

Abstract

We intend (1) to establish the molecular range of modifications to protein integrity and protein structure as such protein becomes associated with mineral surfaces and (2) to determine how mechanisms of protein fragmentation and disintegration, such as oxidation and hydrolysis, depend on mineral surface type and on the conditions in the solution. The latter will be tested by reacting a model protein weight (Gb1 IEP 4.0 6.2 kDA) with a variety of functionally different mineral surfaces (Fe-oxide, montmorillonite, kaolinite and Mn-oxide) at neutral and acidic pH. These systems will subsequently be extracted using a recently developed protocol for metaproteomics analysis (SDS-TCA; Chourey et al. 2010). Consecutive mass - spectrometric and nuclear magnetic resonance analysis conducted at EMSL will allow us to (i) determine if pedogenic oxides will generate more extensive protein alteration than phyllosilicates. We will also determine (ii) how different minerals vary in their ability to induce total or partial fragmentation of the protein. Finally (iii), we will attempt to characterize any structural change intact proteins may exhibit after desorption, the latter assuming that intact proteins are found in the extract. The proposed research will provide insight on protein-matrix interactions and help to constrain enzymatic controls on the production of bioavailable C in terrestrial systems. At the same time, we expect to make a significant contribution to the improvement of protein extraction protocols for metaproteomics

Project Details

Project type
Exploratory Research
Start Date
2013-12-02
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Markus Kleber
Institution
Oregon State University

Team Members

Trang Nguyen
Institution
Oregon State University

Shannon Andrews
Institution
Oregon State University

Thomas Wanzek
Institution
Oregon State University

Kristyn Roscioli
Institution
Pacific Northwest National Laboratory

Stephany Chacon
Institution
Lawrence Berkeley National Laboratory

Related Publications

Chacon S.S., P.N. Reardon, C.J. Burgess, S.O. Purvine, R.K. Chu, T.R. Clauss, and E.D. Walter, et al. 2019. "Mineral Surfaces as Agents of Environmental Proteolysis: Mechanisms and Controls." Journal of Environmental Science and Technology 53, no. 6:3018–3026. PNNL-SA-141783. doi:10.1021/acs.est.8b05583
Reardon PN, SS Chacon, ED Walter, ME Bowden, NM Washton, and MW Kleber. 2016. "Abiotic protein fragmentation by manganese oxide: implications for a mechanism to supply soil biota with oligopeptides." Environmental Science & Technology 50(7):3486–3493. doi:10.1021/acs.est.5b04622