Integrated genomic/transcriptomic/secretomic study of plant-fungal interactions between pines and their symbiotic ectomycorrhizal fungi in the mushroom genus Suillus
EMSL Project ID
48480
Abstract
We propose an integrated -omic study of symbiotic interactions between pines (Pinus spp.) and their ectomycorrhizal fungi (EMF) in the genus Suillus. We are developing the Pine-Suillus symbiosis as a model for understanding EME function across diverse pine-dominated forests of North America, including how symbiotic fungi utilize and sequester plant-derived carbon. As the dominant community of symbiotic microbes in forest soils, EMF atransfer nutrients to tree roots and receive plant carbon in exchange. Suillus species are keystone members of the EMF community that exhibit strong host-specific associations with different Pinus spp. This project will combine study of the fungal genome (by JGI), the mycorrhizal transcriptome (by JGI) and proteome/metabolome (by EMSL) of symbiotic fungal/plant pairs to identify genes/transcripts/proteins/metabolites associated with fungal-plant interaction, nutrient acquisition strategies of Suillus, and host-specificity between species of Suillus and Pinus. By simultaneously exploring the biochemistry of the plant-fungal interface and the fungal-soil interface, results from this study will address the full capacity of Pinus and Suillus species to influence carbon cycling and energy flow in ecosystems.
Project Details
Project type
FICUS Research
Start Date
2014-10-01
End Date
2017-03-31
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members