Correlative Imaging and Spectroscopy of Biological Systems and Structural Dynamics
EMSL Project ID
48671
Abstract
The overall objective of this project is to create an integrative capability for combining dynamic transmission electron microscopy with femtosecond x-ray diffraction, in situ and automated secondary ion mass spectrometry and atom probe tomography to interrogate the structure and dynamics of biological systems. We will develop, adapt, and employ these state-of-the-art approaches to observe biological systems across multiple platforms with various spatial, temporal, and chemical resolutions. Key scientific ideas underlying the proposal involve: (a) assessing diffract-and-destroy compatibility with micro- and nanosecond single-shot electron pulses and femtosecond x-ray pulses; (b) acquiring movies of biological processes in real-time including conformational changes upon ligand binding and assembly/disassembly mechanisms; (c) designing and fabricating novel in situ flow cells that are more versatile for cross-platform analysis with electrons, x-rays and ions; (d) adapting nano-secondary ion mass spectrometry for robust serial section analysis of cells and tissues to provide 3-D chemical information; and (e) establish methodology (sample preparation and parameter optimization) to accurately reconstruct data of biomaterials using atom probe tomography. While the femtosecond x-ray diffraction will be performed at the Linac Coherent Light Source in California (already awarded 60 hours of LCLS beam time in 2013), all other instruments necessary for the success of this research are located at EMSL. The outcome of this research will be a centralized methodology that couples an array of advanced instrumentation techniques to obtain high spatial, chemical, and temporal resolution data of biological processes. The planned integration of imaging and spectroscopy platforms will yield a novel capability at EMSL for true multiscale and multimodal analysis and will enable a better understanding of many biological systems including biofilm organization, enzymatic energy transduction, and epithelial cell interactions with nanomaterial. Furthermore, the research will result in high-quality journal publications of both fundamental knowledge and state-of-the-art imaging technologies using facilities and equipment available at Environmental Molecular Sciences Laboratory.
Project Details
Start Date
2014-11-17
End Date
2015-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Hua X ,Szymanski C J,Wang Z ,Zhou Y ,Ma X ,Yu J ,Evans J E,Orr G ,Liu S ,Zhu Z ,Yu XY 2016. "Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy" Integrative Biology 8(5):579-666. 10.1039/c5ib00308c
Yu X, J Yu, Y Zhou, Y Zhang, J Wang, JE Evans, XY Yu, X Wang, and Z Zhu. 2017. "An Investigation of the Beam Damage Effect on In situ Liquid Secondary Ion Mass Spectrometry Analysis." Rapid Communications in Mass Spectrometry 31(23):2035-2042. doi:10.1002/rcm.7983