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Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework


EMSL Project ID
48672

Abstract

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are involved in metabolic processes, determining how compartments are assembled and how proteins are spatially regulated to optimize their function remains mostly obscure. Imaging technologies are ideally suited to investigate spatially regulated processes and can reveal information from the atomic scale to the mesoscale, depending on the particular technology. However, assimilating data from a multitude of separately optimized approaches comprising different data structures to yield generalizable and fundamental knowledge about a particular biophysical/biochemical process is particularly challenging. We propose to meet this challenge by building a multimodal imaging platform that combines a number of different in-situ imaging modalities with unique chemical probes to quantify the dynamics of cellular processes in unprecedented detail. To drive the development of this imaging platform, we will investigate the response of the growing fungal hyphae tip to nutrient changes.

Project Details

Start Date
2014-11-03
End Date
2015-09-30
Status
Closed

Team

Principal Investigator

James Evans
Institution
Environmental Molecular Sciences Laboratory

Co-Investigator(s)

Aaron Wright
Institution
Pacific Northwest National Laboratory

Team Members

Irina El Khoury
Institution
Environmental Molecular Sciences Laboratory

Chuck Smallwood
Institution
Environmental Molecular Sciences Laboratory

Erin Bredeweg
Institution
Environmental Molecular Sciences Laboratory

Joshua Rosnow
Institution
Pacific Northwest National Laboratory

Kyle Pomraning
Institution
Pacific Northwest National Laboratory

Trevor Moser
Institution
Environmental Molecular Sciences Laboratory

Matthew Marshall
Institution
Pacific Northwest National Laboratory

Ryan Kelly
Institution
Brigham Young University

H Wiley
Institution
Environmental Molecular Sciences Laboratory

Scott Baker
Institution
Environmental Molecular Sciences Laboratory

Galya Orr
Institution
Environmental Molecular Sciences Laboratory

William Cannon
Institution
Pacific Northwest National Laboratory

Related Publications

Geng T, EL Bredeweg, CJ Szymanski, B Liu, SE Baker, G Orr, JE Evans, and RT Kelly. 2015. "Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics." Scientific Reports 5(16111):1-12. doi:10.1038/srep16111
Hua X ,Szymanski C J,Wang Z ,Zhou Y ,Ma X ,Yu J ,Evans J E,Orr G ,Liu S ,Zhu Z ,Yu XY 2016. "Chemical imaging of molecular changes in a hydrated single cell by dynamic secondary ion mass spectrometry and super-resolution microscopy" Integrative Biology 8(5):579-666. 10.1039/c5ib00308c
Liu Y, JK Fredrickson, NC Sadler, P Nandhikonda, RD Smith, and AT Wright. 2015. "Advancing Understanding of Microbial Bioenergy Conversion Processes by Activity-Based Protein Profiling." Biotechnology for Biofuels 8(156):, doi:10.1186/s13068-015-0343-7