Dissection and Deciphering of the Soil Microbiome
EMSL Project ID
48784
Abstract
This proposal aims to address key gaps in our knowledge about the composition and functional roles of the tens of thousands of microbial species in soil. Although these microorganisms carry out critical processes for life on earth, including cycling of nutrients and decomposition of organic matter, the majority (>90%) have never been cultivated and their identities and functions are unknown. By application of new molecular “omics” tools it has been possible to obtain sequence and protein data from some soils. However, due to the high microbial diversity in soil, the data is extremely challenging to analyze and interpret. Therefore, we aim to take a novel approach to dissect the soil community in order to reduce the microbial diversity prior to analysis by sequencing and other molecular approaches to enable deciphering of the identities and key roles played by select members of the soil microbiome. This information will further facilitate molecular screening of the entire soil microbial community by use of the select genome data as a scaffold for aid in interpretation of the large data sets.
Project Details
Start Date
2015-03-02
End Date
2015-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Koeberl M, RA White, III, S Erschen, N Spanberger, TF El-Arabi, JK Jansson, and G Berg. 2015. "Complete genome sequence of Bacillus amyloliquefaciens strain Co1-6, a plant growth-promoting rhizobacterium of Calendula officinalis." Genome Announcements 3(4):Article No. e00862-15. doi:10.1128/genomeA.00862-15
Koeberl M, RA White, III, S Erschen, TF El-Arabi, JK Jansson, and G Berg. 2015. "Draft genome sequence of Paenbacillus polymyxa strain Mc5Re-14, an antagonistic root endophyte of Matricaria chamomilla." Genome Announcements 3(4):Article No. e00861-15. doi:10.1128/genomeA.00861-15
Koeberl M, RA White, III, S Erschen, TF El-Arabi, JK Jansson, and G Berg. 2015. "Draft genome sequence of Streptomyces sp. strain Wb2n-11, a desert isolate with broad-spectrum antagonism against soilborne phytopathogens ." Genome Announcements 3(4):e00860-15. doi:10.1128/genomeA.00860-15
Roy Chowdhury T., J. Lee, E.M. Bottos, C.J. Brislawn, R.A. White, L.M. Bramer, and J.M. Brown, et al. 2019. "Metaphenomic responses of a native prairie soil microbiome to moisture perturbations." mSystems 4, no. 4:e00061-19. PNNL-SA-129510. doi:10.1128/mSystems.00061-19