Development of an in situ multimodal mass spectrometry imaging approach for studying the rhizosphere: from revealing activation of metabolic pathways to elucidating nanoscale exchanges
EMSL Project ID
49044
Abstract
Gaining spatial information of biochemical interactions that occur within the rhizosphere and other subsurface terrestrial ecosystems is an area of major interest. Through bulk extraction methods, we have been able to demonstrate major differences in the molecular makeup of these systems, achieving detection and classification of every major class of biomolecules. However, the ability to visualize molecular distribution at small spatial scales, and in an in situ manner, has remained elusive. Here, we propose to develop ambient MSI approaches for in situ molecular imaging of the rhizosphere. These will be the first steps in our “top-down” multimodal MSI approach, where we seek to understand milli- to sub-micrometer interactions of rhizospheric systems by chemical imaging.
Project Details
Start Date
2015-10-23
End Date
2017-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Velickovic D, RK Chu, AA Carrell, M Thomas, L Pasa Tolic, DJ Weston, and CR Anderton. 2018. "Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Con?dence in Both Molecular Identi?cation and Localization." Analytical Chemistry 90(1):702-707. doi:10.1021/acs.analchem.7b04319
Velickovic DS, and CR Anderton. 2017. "Mass Spectrometry Imaging: Towards Mapping The Elemental And Molecular Composition Of The Rhizosphere." Rhizosphere 3(Part 2):254-258. doi:10.1016/j.rhisph.2017.03.003