A Molecular Atlas of the Developing Lung
EMSL Project ID
49133
Abstract
The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step toward promoting proper lung formation in preterm infants. This, however, remains an unsolved challenge as no systematic study of the molecular components of normal lung development during late term and early childhood has previously been conducted. Within organs such as the lung, the relationship between space, anatomy, and function is fundamental. Therefore, our approach will include imaging techniques with high spatial resolution, as well as cell-specific 'omics. The future correlation of these complementary data collection methods will facilitate the establishment of cell-specific spatial informatics across the developing lung. Specifically, we will accomplish our goal of an integrated molecular atlas of lung development through the following aims: (1) Spatial imaging for a molecular atlas of the developing mouse and human lung (2) Cell type specific omics for a molecular atlas of the developing mouse and human lung (3) Manage data and metadata to facilitate collaboration and data integration. Overall, these aims will create the first spatial-temporal molecular atlas of the mammalian lung during alveologenesis, which in coordination with the other LungMAP centers will provide an unprecedented array of information about the healthy developing lungs in mouse and human. The novel imaging approaches and the suite of integrated pan-omics capabilities developed and available in a single laboratory at PNNL represents a unique strength of the Research Center.
Project Details
Start Date
2015-10-22
End Date
2016-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Clair GCD, PD Piehowski, T Nicola, J Kitzmiller, EL Huang, EM Zink, RL Sontag, DJ Orton, RJ Moore, JP Carson, RD Smith, J Whitsett, RA Corley, N Ambalavanan, and CK Ansong. 2016. "Spatially-Resolved Proteomics: Rapid Quantitative Analysis of Laser Capture Microdissected Alveolar Tissue Samples." Scientific Reports 6:Article No. 39223. doi:10.1038/srep39223
Dautel SE, Kyle JE, Clair G, Sontag RL, Weitz KK, Shukla AK, Nguyen SN, Kim YM, Zink EM, Luders T, Frevert CW, Gharib SA, Laskin J, Carson JP, Metz TO, Corley RA, Ansong C."Lipidomics reveals dramatic lipid compositional changes in the maturing postnatal lung."
Sci Rep. 2017 Feb 1;7:40555. doi: 10.1038/srep40555.
Nguyen SN, RL Sontag, JP Carson, RA Corley, CK Ansong, and J Laskin. 2018. "Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy." Journal of the American Society for Mass Spectrometry 29(2):316-322. doi:10.1007/s13361-017-1750-8