Skip to main content

Connecting genomic capabilities to physiology, photosynthesis and carbon cycling


EMSL Project ID
49288

Abstract

More than 50% of global primary production is performed by land plants and green algae. Of all algae green algae are the most closely related to land plants and therefore provide powerful single cell systems for exploring how 'plant' genes link to physiology and the photosynthetic processes that underlie primary production. Prasinophyte green algae are also widespread in the marine environment, and the genus Micromonas is found from the tropics into polar waters. This project aims to understand plant and algal physiology using Micromonas as a model. Specifically, we aim to connect genes with processes and interactions, photosynthesis and stress, alongside increased CO2 levels. Samples will be analyzed from systems biology studies aimed to efficiently address environmental impacts on primary producers. Using peptide analysis, metabolomics and lipid measurements as well as elemental analysis we aim to improve metabolic mapping within the cell and to connect proteins of unknown function with growth and stress responses in an important marine photosynthetic microbial eukaryote that also sheds light on the biology of land plants.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2016-10-01
End Date
2018-09-30
Status
Closed

Team

Principal Investigator

Alexandra Worden
Institution
Monterey Bay Aquarium Research Institute

Co-Investigator(s)

Stephen Callister
Institution
Pacific Northwest National Laboratory

Team Members

Richard Smith
Institution
Pacific Northwest National Laboratory

Related Publications

Guo J., S. Wilken, V. Jimenez, C. Jae Choi, C.K. Ansong, R.O. Dannebaum, and L. Sudek, et al. 2018. "Specialized proteomic responses and an ancient photoprotection mechanism sustain marine green algal growth during phosphate limitation." Nature Microbiology 3, no. 7:781-790. PNNL-SA-135227. doi:10.1038/s41564-018-0178-7