Identification of Tumor Viruses using DTA and Proteomics
EMSL Project ID
49594
Abstract
The Chang/Moore laboratory, in collaboration with PNNL, was awarded new funding to pursue the discovery of novel cancer viruses in hematologic malignancies using a combination of genomic and proteomic approaches for both directed and unbiased data generation and comparison. The directed approach will survey a large panel of hematologic malignancies for the presence of the new human polymaviruses. The unbiased approach will use DTS to examine highly-selected gold-standard cases of EBV-negative post-transplant lymphoproliferative disorder (EN-PTLD) for presence of novel viral transcripts. High PHRED-equivalent sequencing of EN-PTLD tumors will be performed to identify novel viral transcripts. These data will be compared to DTS results on EBV-positive PTLD to confirm virus transcript detection and determine differential cellular gene expression patterns between EBV-negative and positive disease. Specific to PNNL’s contribution to this collaborative project, application of advanced LC-MS/MS proteomics capabilities at EMSL will be applied to proteins extracted from EN-PTLD tissue samples with comparison of DTS transcripts and detected peptide sequence data from the same tissue samples that will allow a more precise subtraction of human sequence data in the identification of novel viruses present in tumors.
Project Details
Start Date
2016-10-07
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator