Unraveling New Pathways for Carbon Capture to Enhance Lipid-Biomass Feedstocks
EMSL Project ID
49612
Abstract
Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are involved in metabolic processes, determining how compartments are assembled and how proteins are spatially regulated to optimize their function remains mostly obscure. Imaging technologies are ideally suited to investigate spatially regulated processes and can reveal information from the atomic scale to the mesoscale, depending on the particular technology. However, assimilating data from a multitude of separately optimized approaches comprising different data structures to yield generalizable and fundamental knowledge about a particular biophysical/biochemical process is particularly challenging. We propose to meet this challenge by building a multimodal imaging platform that combines a number of different in-situ imaging modalities with unique chemical probes to quantify the dynamics of cellular processes in unprecedented detail. This work is fully funded by a DOE-BER Mesoscale to Molecules Bioimaging project so only access to the instrumentation is being requested.
Project Details
Start Date
2016-10-06
End Date
2019-12-31
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Casadei C.M., C. Tsai, A. Barty, M. Hunter, N.A. Zatsepin, C. Padeste, and G. Capitani, et al. 2018. "Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals." International Union of Crystallography Journal 5, no. 1:103-117. PNNL-SA-136382. doi:10.1107/S2052252517017043
Gallagher-Jones M., C. Glynn, D.R. Boyer, M.W. Martynowycz, E. Hernandez, J. Miao, and C. Zee, et al. 2018. "Sub-angstrom cryo-EM structure of a prion protofibril reveals a polar clasp." Nature Structural & Molecular Biology 25, no. February 2018:131-134. PNNL-SA-133752. doi:10.1038/s41594-017-0018-0
Geng T., C.R. Smallwood, E.L. Bredeweg, K.R. Pomraning, A.E. Plymale, S.E. Baker, and J.E. Evans, et al. 2017. "Multimodal Microfluidic Platform for Controlled Culture and Analysis of Unicellular Organisms." Biomicrofluidics 11, no. 5:054104. PNNL-SA-129125. doi:10.1063/1.4986533
Henske JK, SE Wilken, KV Solomon, CR Smallwood, V Shutthanandan, JE Evans, MK Theodorou, and MA O'Malley. 2018. "Metabolic characterization of anaerobic fungi provides a path forward for bioprocessing of crude lignocellulose." Biotechnology and Bioengineering 115(4):874-884. doi:10.1002/bit.26515
Moser T.H., H.S. Mehta, C. Park, R.T. Kelly, T. Shokuhfar, and J.E. Evans. 2018. "The role of electron irradiation history in liquid cell transmission electron microscopy." Science Advances 4, no. 4:eaaq1202. PNNL-SA-120688. doi:10.1126/sciadv.aaq1202
Moser T., T. Shokuhfar, and J.E. Evans. 2019. "Considerations for imaging thick, low contrast, and beam sensitive samples with liquid cell transmission electron microscopy." Micron 117. PNNL-SA-136788. doi:10.1016/j.micron.2018.10.007