Agile BioFoundry
EMSL Project ID
49659
Abstract
This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger FY17-19 nine-national laboratory ABF project. PNNL’s effort for these projects, which includes the analytical work proposed in this Resource Owner’s project as well as strain development at the BSEL will support Task 1 of both ABF multi-lab projects. The objective of the multi-lab projects is to establish a distributed Synthetic Biology Foundry (known as the Agile BioFoundry (ABF), through a consortium of nine national laboratories, that will productionize synthetic biology by integrating advanced biological engineering with computing, overall process analysis, and scale-up. This effort will establish a differentiated and unique capability that will enable BETO to meet its goals for more cost-effective production of bio-based fuels and products through a 40% increase in efficiency of biological process development and a 50% reduction in time-to-market.This EMSL project will provide analytical results from leading edge analytical biology, both GC and LC mass spectrometry and 13C metabolic flux analysis (13C MFA). These analytical results are required for monitoring proteins and metabolites for assessing the impact of introducing new pathways into host organisms through identification of host genomic modifications necessary to relieve bottlenecks in the production of adipic acid and other target products of the projects. The analytical results from this EMSL project will provide guidance for the PNNL strain development effort conducted at the BSEL and to the strain development efforts at the partner national laboratories for the two ABF projects. Successful application of the EMSL-generated results will demonstrate the important role that these EMSL analytical capabilities can play in enabling the rapid engineering cycles necessary for competitive development of new production hosts and pathways. This EMSL project will showcase EMSL analytical biology capabilities and demonstrate their usefulness to the broader research community. The PNNL portion of the large multi-lab ABF project also provides an opportunity to demonstrate the benefits merging EMSL and BSEL capabilities for the success of a collaborative research project can afford. It is expected that the results of the ABF project will be of interest to, and will have application for, entities interested in development of production hosts such as enzyme companies, biofuel companies, biochemical companies, academics, and national laboratory scientists. Distribution of the results of this project will be through communications conducted through a variety of channels including technical publications and presentations at conferences, along with direct pursuit of opportunities to explore the potential for collaborations with industrial partners who potentially could apply the findings of this project to their own process development efforts. Engaging industry for technology transfer is written into the operating plan for the ABF project.
Project Details
Start Date
2016-11-17
End Date
2017-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members