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Probing complex microbiomes using mass spectrometry and sequencing capabilities to understand how microbiomes are influenced by their environment


EMSL Project ID
49692

Abstract

This is a resource owner proposal as part of the MinT LDRD Initiative. The objective of this proposal is the development of sequencing and mass spectrometry capabilities to support research into how soil microbiomes respond to their environment.

Project Details

Start Date
2017-01-25
End Date
2017-09-30
Status
Closed

Team

Principal Investigator

Stephen Callister
Institution
Pacific Northwest National Laboratory

Team Members

Eric Bottos
Institution
Pacific Northwest National Laboratory

Taniya Roy Chowdhury
Institution
Woodwell Climate Research Center

Richard White
Institution
Washington State University

Jared Shaw
Institution
Environmental Molecular Sciences Laboratory

Carrie Nicora
Institution
Pacific Northwest National Laboratory

Ljiljana Pasa-Tolic
Institution
Environmental Molecular Sciences Laboratory

Related Publications

Callister S.J., L. McCue, A.A. Boaro, B.L. Lamarche, R.A. White, J.M. Brown, and B.K. Ahring. 2018. "Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor." PLoS One 13, no. 10:Article number e0204831. PNNL-SA-133574. doi:10.1371/journal.pone.0204831
Callister S.J., T.L. Fillmore, C.D. Nicora, J.B. Shaw, S.O. Purvine, D.J. Orton, and R.A. White, et al. 2018. "Addressing the challenge of soil metaproteome complexity by improving metaproteome depth of coverage Through two-dimensional liquid chromatography." Soil Biology and Biochemistry 125. PNNL-SA-131999. doi:10.1016/j.soilbio.2018.07.018