Deciphering electron transfer in biofilm communications by in situ chemical imaging
EMSL Project ID
49694
Abstract
We seek to understand how the dynamics of nutrients, metabolites, and solid surface influence microbial processes in biofilm systems. Our overarching hypothesis is that biophysical and biochemical changes at the molecular level translate to cellular biochemical and structural responses and ultimately determine functions of microbial systems in a specific environment (e.g., biofilms). Chemical and metabolic observations corresponding to structural data are needed for mesoscale studies. We aim to address the following science questions: 1) What are the important molecular processes that control ion/electron transport across biological interfaces? and 2) How does nutrient exchange in a complex ecosystem influence molecular processes and microbial functions? We will conduct an integrative analysis of communication among biological subcellular components and multicellular organisms at scales ranging from molecular to several micrometers relevant to ecosystem levels using the unique PNNL analytical instrument, SAVLI (system for analysis at the liquid vacuum interface) coupled with multiple chemical imaging techniques. The portable SALVI platform, developed in EMSL, is suitable for multiple imaging tools such as time-of-flight secondary ion mass spectrometry (ToF-SIMS) and nuclear magnetic resonance (NMR). Using the unique SALVI microfluidic platform, we will examine how spatial heterogeneity in biofilm formation and EPS production influences biofilm dynamics in the mesoscale by imaging live biofilms using multimodal chemical imaging techniques. We will also investigate electron transfer reactions at the interface between the biofilm and the anode electrode to ascertain how potential-dependent current production varies at different development stages of biofilm from single attached cells to a mature, multicellular, three-dimensional structure. More importantly, interspecies electron transfer will be studied dynamically. These results will provide dynamic multiscale understanding of diverse microbial phenomic parameters from living biofilms and underpin integrative predications of dynamic cellular processes and interactions of real-world environments. The multimodal mesoscale imaging results will advance fundamental understandings of molecular processes in Biosystem Dynamics and Design and Energy Materials and processes. Such fundamental insight is also relevant to Terrestrial and Subsurface Ecosystems.
Project Details
Start Date
2017-02-02
End Date
2017-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
In Vivo Molecular Insights into Syntrophic Geobacter Aggregates
Wei W., A.E. Plymale, Z. Zhu, X. Ma, F. Liu, and X. Yu. 2020. "In Vivo Molecular Insights into Syntrophic Geobacter Aggregates." Analytical Chemistry 92, no. 15:10402-10411. PNNL-SA-151938. doi:10.1021/acs.analchem.0c00653
Characterization of syntrophicGeobactercommunities using ToF-SIMS
Wei W., R.E. Komorek, Y. Zhang, A.E. Plymale, R. Yu, B. Wang, and Z. Zhu, et al. 2017. "Characterization of Syntrophic Geobacter Communities Using ToF-SIMS." Biointerphases 12, no. 5:05G601 1-11. PNNL-SA-125721. doi:10.1116/1.4986832
Revealing the Structural Evolution of Green Rust Synthesized in Ionic Liquids by In Situ Molecular Imaging
Shen Y., Y. Fu, J. Yao, D.B. Lao, S.K. Nune, Z. Zhu, and D.J. Heldebrant, et al. 2020. "Revealing the structural evolution of green rust synthesized in ionic liquids by in situ molecular imaging." Advanced Materials Interfaces. PNNL-SA-151430. doi:10.1002/admi.202000452
Does interfacial photochemistry play a role in the photolysis of pyruvic acid in water?
Fu Y., Y. Zhang, F. Zhang, J. Chen, Z. Zhu, and X. Yu. 2018. "Does interfacial photochemistry play a role in the photolysis of pyruvic acid in water?." Atmospheric Environment 191. PNNL-SA-137894. doi:10.1016/j.atmosenv.2018.07.061
Superparamagnetic CoFe2O4@Au with High Specific Absorption Rate and Intrinsic Loss Power for Magnetic Fluid Hyperthermia Applications
Sabale S.R., V. Jadhav, S.J. Mane-Gavade, and X. Yu. 2019. "Superparamagnetic CoFe2O4@Au with high specific absorption rate and intrinsic loss power for magnetic fluid hyperthermia applications." Acta Meteorologica Sinica 32, no. 6:719-725. PNNL-SA-125714. doi:10.1007/s40195-018-0830-5
<em>In Situ</em> Characterization of Boehmite Particles in Water Using Liquid SEM
Yao J, BW Arey, L Yang, F Zhang, RE Komorek, J Chun, and XY Yu. 2017. "In Situ Characterization of Boehmite Particles in Water Using Liquid SEM." Journal of Visualized Experiments (127):Video No. e56058. doi:10.3791/56058
<em>In Situ</em> Characterization of <em>Shewanella oneidensis</em> MR1 Biofilms by SALVI and ToF-SIMS
Komorek RE, W Wei, X Yu, EA Hill, J Yao, Z Zhu, and XY Yu. 2017. "In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS." Journal of Visualized Experiments (126):Article No. e55944. doi:10.3791/55944
Deciphering the aqueous chemistry of glyoxal oxidation with hydrogen peroxide using molecular imaging
Sui X, Y Zhou, F Zhang, J Chen, Z Zhu, and XY Yu. 2017. "Deciphering the Aqueous Chemistry of Glyoxal Oxidation with Hydrogen Peroxide Using Molecular Imaging." Physical Chemistry Chemical Physics. PCCP 19(31):20357-20366. doi:10.1039/C7CP02071F
Two coexisting liquid phases in switchable ionic liquids
Yao J, D Lao, X Sui, Y Zhou, SK Nune, X Ma, T Troy, M Ahmed, Z Zhu, DJ Heldebrant, and XY Yu. 2017. "Two Coexisting Liquid Phases in Switchable Ionic Liquids." Physical Chemistry Chemical Physics. PCCP 19(34):22627-22623. doi:10.1039/C7CP03754F
<em>In Situ</em> Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
Yu J, Y Zhou, X Hua, Z Zhu, and XY Yu. 2016. "In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS ." Journal of Visualized Experiments (108):Video No. e53708. doi:10.3791/53708
In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel
Renslow RS, MJ Marshall, AE Tucker, WB Chrisler, and XY Yu. 2017. "In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel." Analyst 142(13):2363-2371. doi:10.1039/C7AN00078B