Deciphering electron transfer in biofilm communications by in situ chemical imaging
EMSL Project ID
49694
Abstract
We seek to understand how the dynamics of nutrients, metabolites, and solid surface influence microbial processes in biofilm systems. Our overarching hypothesis is that biophysical and biochemical changes at the molecular level translate to cellular biochemical and structural responses and ultimately determine functions of microbial systems in a specific environment (e.g., biofilms). Chemical and metabolic observations corresponding to structural data are needed for mesoscale studies. We aim to address the following science questions: 1) What are the important molecular processes that control ion/electron transport across biological interfaces? and 2) How does nutrient exchange in a complex ecosystem influence molecular processes and microbial functions? We will conduct an integrative analysis of communication among biological subcellular components and multicellular organisms at scales ranging from molecular to several micrometers relevant to ecosystem levels using the unique PNNL analytical instrument, SAVLI (system for analysis at the liquid vacuum interface) coupled with multiple chemical imaging techniques. The portable SALVI platform, developed in EMSL, is suitable for multiple imaging tools such as time-of-flight secondary ion mass spectrometry (ToF-SIMS) and nuclear magnetic resonance (NMR). Using the unique SALVI microfluidic platform, we will examine how spatial heterogeneity in biofilm formation and EPS production influences biofilm dynamics in the mesoscale by imaging live biofilms using multimodal chemical imaging techniques. We will also investigate electron transfer reactions at the interface between the biofilm and the anode electrode to ascertain how potential-dependent current production varies at different development stages of biofilm from single attached cells to a mature, multicellular, three-dimensional structure. More importantly, interspecies electron transfer will be studied dynamically. These results will provide dynamic multiscale understanding of diverse microbial phenomic parameters from living biofilms and underpin integrative predications of dynamic cellular processes and interactions of real-world environments. The multimodal mesoscale imaging results will advance fundamental understandings of molecular processes in Biosystem Dynamics and Design and Energy Materials and processes. Such fundamental insight is also relevant to Terrestrial and Subsurface Ecosystems.
Project Details
Start Date
2017-02-02
End Date
2017-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Fu Y., Y. Zhang, F. Zhang, J. Chen, Z. Zhu, and X. Yu. 2018. "Does interfacial photochemistry play a role in the photolysis of pyruvic acid in water?." Atmospheric Environment 191. PNNL-SA-137894. doi:10.1016/j.atmosenv.2018.07.061
Komorek RE, W Wei, X Yu, EA Hill, J Yao, Z Zhu, and XY Yu. 2017. "In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS." Journal of Visualized Experiments (126):Article No. e55944. doi:10.3791/55944
Renslow RS, MJ Marshall, AE Tucker, WB Chrisler, and XY Yu. 2017. "In situ nuclear magnetic resonance microimaging of live biofilms in a microchannel." Analyst 142(13):2363-2371. doi:10.1039/C7AN00078B
Sabale S.R., V. Jadhav, S.J. Mane-Gavade, and X. Yu. 2019. "Superparamagnetic CoFe2O4@Au with high specific absorption rate and intrinsic loss power for magnetic fluid hyperthermia applications." Acta Meteorologica Sinica 32, no. 6:719-725. PNNL-SA-125714. doi:10.1007/s40195-018-0830-5
Shen Y., Y. Fu, J. Yao, D.B. Lao, S.K. Nune, Z. Zhu, and D.J. Heldebrant, et al. 2020. "Revealing the structural evolution of green rust synthesized in ionic liquids by in situ molecular imaging." Advanced Materials Interfaces. PNNL-SA-151430. doi:10.1002/admi.202000452
Sui X, Y Zhou, F Zhang, J Chen, Z Zhu, and XY Yu. 2017. "Deciphering the Aqueous Chemistry of Glyoxal Oxidation with Hydrogen Peroxide Using Molecular Imaging." Physical Chemistry Chemical Physics. PCCP 19(31):20357-20366. doi:10.1039/C7CP02071F
Wei W., A.E. Plymale, Z. Zhu, X. Ma, F. Liu, and X. Yu. 2020. "In Vivo Molecular Insights into Syntrophic Geobacter Aggregates." Analytical Chemistry 92, no. 15:10402-10411. PNNL-SA-151938. doi:10.1021/acs.analchem.0c00653
Wei W., R.E. Komorek, Y. Zhang, A.E. Plymale, R. Yu, B. Wang, and Z. Zhu, et al. 2017. "Characterization of Syntrophic Geobacter Communities Using ToF-SIMS." Biointerphases 12, no. 5:05G601 1-11. PNNL-SA-125721. doi:10.1116/1.4986832
Wei W, Y Zhang, RE Komorek, AE Plymale, B Wang, R Yu, F Liu, Z Zhu, and XY Yu. 2017. "Characterization of syntrophic Geobacter communities using ToF-SIMS." Biointerphases. [In Press]
Yao J, BW Arey, L Yang, F Zhang, RE Komorek, J Chun, and XY Yu. 2017. "In Situ Characterization of Boehmite Particles in Water Using Liquid SEM." Journal of Visualized Experiments (127):Video No. e56058. doi:10.3791/56058
Yao J, D Lao, X Sui, Y Zhou, SK Nune, X Ma, T Troy, M Ahmed, Z Zhu, DJ Heldebrant, and XY Yu. 2017. "Two Coexisting Liquid Phases in Switchable Ionic Liquids." Physical Chemistry Chemical Physics. PCCP 19(34):22627-22623. doi:10.1039/C7CP03754F
Yu J, Y Zhou, X Hua, Z Zhu, and XY Yu. 2016. "In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS ." Journal of Visualized Experiments (108):Video No. e53708. doi:10.3791/53708