Integrated multi-omic analysis of high lipid accumulation in the oleaginous green microalgae Ostreococcus tauri to engineer metabolic pathways for improved triacylglycerol feedstocks
EMSL Project ID
50036
Abstract
Our proposed mutli-omic approach will empower a comprehensive understanding of cellular pathways linking carbon, nitrogen and phosphate metabolism in the microalga Ostreococcus tauri. The use of this highly simplified and smallest free-living photoautotrophic eukaryote helps to ensure a positive outcome of the research by providing a more compact template with fewer redundant pathways while retaining the same mechanisms employed by higher eukaryotes. As such our results will resolve regulatory relationships in the ancient O. tauri cell and will also be directly extensible to a wide-range of organisms of prime interest to BER. Integrating metabolomic, proteomic, and lipidomic data acquired under this proposal with chemical, structural and dynamic imaging from a companion proposal will help identify specific metabolic pathways and regulatory elements that can be exploited to guide future synthetic biology efforts to design even more efficient organisms for bioenergy applications.
Project Details
Project type
Exploratory Research
Start Date
2017-11-29
End Date
2018-12-31
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members