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Integrated multi-omic analysis of high lipid accumulation in the oleaginous green microalgae Ostreococcus tauri to engineer metabolic pathways for improved triacylglycerol feedstocks


EMSL Project ID
50036

Abstract

Our proposed mutli-omic approach will empower a comprehensive understanding of cellular pathways linking carbon, nitrogen and phosphate metabolism in the microalga Ostreococcus tauri. The use of this highly simplified and smallest free-living photoautotrophic eukaryote helps to ensure a positive outcome of the research by providing a more compact template with fewer redundant pathways while retaining the same mechanisms employed by higher eukaryotes. As such our results will resolve regulatory relationships in the ancient O. tauri cell and will also be directly extensible to a wide-range of organisms of prime interest to BER. Integrating metabolomic, proteomic, and lipidomic data acquired under this proposal with chemical, structural and dynamic imaging from a companion proposal will help identify specific metabolic pathways and regulatory elements that can be exploited to guide future synthetic biology efforts to design even more efficient organisms for bioenergy applications.

Project Details

Project type
Exploratory Research
Start Date
2017-11-29
End Date
2018-12-31
Status
Closed

Team

Principal Investigator

James Evans
Institution
Environmental Molecular Sciences Laboratory

Team Members

Chuck Smallwood
Institution
Environmental Molecular Sciences Laboratory

Ryan Kelly
Institution
Brigham Young University

William Cannon
Institution
Pacific Northwest National Laboratory