Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions
EMSL Project ID
50079
Abstract
Microbes orchestrate a suite of intra- and inter-cellular biomolecules to carry out diverse metabolic processes. Existing genomic and biochemical methods cannot directly probe the physical connections involved in such processes over the relevant length scales, spanning the nano-meso-micrometer spatial regimes. Determining the location and function of such biomolecules would aid in identifying the mechanisms governing microbial interactions. Furthermore, the chemical identities of the components driving the flow of materials in complex microbial communities are largely unknown. We will address these technical and conceptual gaps by developing an innovative single transferable multimodal chemical imaging platform, Chemical Nanoscope, that can interrogate biomolecules in living systems using three complementary label-free, nanoscale, ultrasensitive chemical imaging techniques:1. Infrared scattering scanning near-field optical microscopy (IR s-SNOM)
2. Tip-enhanced Raman nano-spectroscopy (TERS)
3. Tip-enhanced UV-Vis-Near-IR optical absorption nano-spectroscopy (TAN).
This unique atomic force microscopy (AFM) -based instrument will be used to investigate a wide range of biomolecules (nucleic acids, proteins, lipids, polysaccharides, biological chromophores) through their characteristic electronic (TAN) and vibrational (IR s-SNOM and TERS) signatures, over the nano-meso-micrometer length scales. Our platform will not only enable recording chemical images of single microbial cells with subcellular resolution (~10 nm), but it will also enable mapping an entire microbial community, or “microbiome”, (~100s of microns) with chemical selectivity.
Project Details
Start Date
2017-11-10
End Date
2020-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Apra E., A. Bhattarai, K.T. Crampton, E.J. Bylaska, N. Govind, W.P. Hess, and P.Z. El-Khoury. 2018. "Time Domain Simulations of Single Molecule Raman Scattering." Journal of Physical Chemistry A 122, no. 37:7437-7442. PNNL-SA-135554. doi:10.1021/acs.jpca.8b05912
Bhattarai A., A.G. Joly, A.V. Krayev, and P.Z. El-Khoury. 2019. "Taking the Plunge: Nanoscale Chemical Imaging of Functionalized Gold Triangles in H2O via TERS." Journal of Physical Chemistry C 123, no. 12:7376-7380. PNNL-SA-140448. doi:10.1021/acs.jpcc.9b00867
Bhattarai A., A.G. Joly, W.P. Hess, and P.Z. El-Khoury. 2017. "Visualizing Electric Fields at Au(111) Step Edges via Tip-Enhanced Raman Scattering." Nano Letters 17, no. 11:7131-7137. PNNL-SA-129323. doi:10.1021/acs.nanolett.7b04027
Bhattarai A., and P.Z. El-Khoury. 2019. "Nanoscale Chemical Reaction Imaging at the Solid-Liquid Interface via TERS." Journal of Physical Chemistry Letters 10, no. 11:2817-2822. PNNL-SA-141439. doi:10.1021/acs.jpclett.9b00935
Bhattarai A., A.V. Krayev, A. Temiryazev, D. Evplov, K.T. Crampton, W.P. Hess, and P.Z. El-Khoury. 2018. "Tip-Enhanced Raman Scattering from Nanopatterned Graphene and Graphene Oxide." Nano Letters 18, no. 6:4029-4033. PNNL-SA-134494. doi:10.1021/acs.nanolett.8b01690
Bhattarai A., K.T. Crampton, A.G. Joly, L. Kovarik, W.P. Hess, and P.Z. El-Khoury. 2018. "Imaging the Optical Fields of Functionalized Silver Nanowires through Molecular TERS." The Journal of Physical Chemistry Letters 9, no. 24:7105-1709. PNNL-SA-139471. doi:10.1021/acs.jpclett.8b03324
O'Callahan B.T., K. Park, I.V. Novikova, T. Jian, C. Chen, E.A. Muller, and P.Z. El-Khoury, et al. 2020. "In liquid infrared scattering scanning near-field optical microscopy for chemical and biological nanoimaging." Nano Letters 20, no. 6:4497-4504. PNNL-SA-152805. doi:10.1021/acs.nanolett.0c01291
O'Callahan B.T., K.T. Crampton, I.V. Novikova, T. Jian, C. Chen, J.E. Evans, and M.M. Raschke, et al. 2018. "Imaging Nanoscale Heterogeneity in Ultrathin Biomimetic and Biological Crystals." Journal of Physical Chemistry C 122, no. 43:24891-24895. PNNL-SA-136203. doi:10.1021/acs.jpcc.8b06681
O'Callahan B.T., K.T. Crampton, I.V. Novikova, T. Jian, C. Chen, J.E. Evans, and M.M. Raschke, et al. 2018. "Imaging Nanoscale Heterogeneity in Ultrathin Biomimetic and Biological Crystals." Journal of Physical Chemistry C. doi:10.1021/acs.jpcc.8b06681.
O'Callahan B.T., M. Hentschel, M.B. Raschke, P.Z. El-Khoury, and A. Lea. 2019. "Ultrasensitive Tip- and Antenna-Enhanced Infrared Nanoscopy of Protein Complexes." Journal of Physical Chemistry C 123, no. 28:17505-17509. PNNL-SA-144661. doi:10.1021/acs.jpcc.9b05777