Combining surface analysis and mass spectrometry methods to expand the information available from biologically and environmentally relevant interfaces
EMSL Project ID
50150
Abstract
EMSL and the NESAC/BIO (National Electron Spectroscopy Analysis Center for Biology) at the University of Washington are world leaders in the application of surface analysis and mass spectrometry to a wide range of important scientific and societal problems. The instrumentation and expertise of the two world leading capabilities are complementary. The UW center, currently led by Dave Castner and Lara Gamble, has pioneered the application of surface methods to protein surfaces and other biological interfaces. EMSL has significantly greater capabilities in mass spectrometry than the NESAC/BIO facility and complementary expertise in XPS and TOF-SIMS. This staff proposal continues ongoing collaborations that seek to expand and advance the quantification of information that can be extracted from biological and environmental systems with a focus on interfaces and small volume high resolution analysis. The specific areas of current development include advancing the ability XPS to quantify organic coatings on particles of biological and environmental relevance, including those formed on nanoparticles, and application of MALDI-MS/MS to complement TOF-SIMS localized measurements linking to the established LC-MS/MS data streams available at PNNL to expand the molecular level information available.
Project Details
Start Date
2018-02-21
End Date
2020-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Angerer T.B., D. Velickovic, C.D. Nicora, J.E. Kyle, D.J. Graham, C.R. Anderton, and L.J. Gamble. 2019. "Exploiting the Semidestructive Nature of Gas Cluster Ion Beam Time-of-Flight Secondary Ion Mass Spectrometry Imaging for Simultaneous Localization and Confident Lipid Annotations." Analytical Chemistry 91, no. 23:15073-15080. PNNL-SA-150104. doi:10.1021/acs.analchem.9b03763
Angerer T.B., N. Chakravarty, M.J. Taylor, M.J. Taylor, C.D. Nicora, D.J. Graham, and C.R. Anderton, et al. 2019. "Insights into the histology of planarian flatworm Phagocata gracilis based on location specific, intact lipid information provided by GCIB-ToF-SIMS imaging." Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids. 1864, no. 5:733-743. PNNL-SA-140046. doi:10.1016/j.bbalip.2019.01.011
Shah D., D.I. Patel, T. Roychowdhury, G.B. Rayner, N. O'Toole, D.R. Baer, and M.R. Linford. 2018. "A Tutorial on Interpreting X-ray Photoelectron Spectroscopy (XPS) Survey." Journal of Vacuum Science and Technology B--Microelectronics and Nanometer Structures 36, no. 6:062902. PNNL-SA-138872. doi:10.1116/1.5043297