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Solid-state NMR Investigation into the Transformation of Necromass to SOM


EMSL Project ID
50153

Abstract

NMR will be utilized to help bridge the knowledge gap between carbon cycling and storage of carbon within soil organic matter (SOM). Specifically, NMR will help address the questions: (1) how much organic carbon enters the soil; (2) where does it enter the soil; (3) what is the composition of the organic matter input; (4) how do plants and microbes influence compounds derived from the each other. To address these questions, the extracellular, intracellular and the insoluble fractions of microbial biomass that we hypothesize will become necromass within soil will be analyzes using NMR. Sub-communities from Michigan and Wisconsin whole soils (GLBRC) have been enriched and cultured in order to to describe the chemical fingerprints of extracellular, intracellular and insoluble fractions of microbial biomass across different bacterial and fungal phylotypes. Molecular signatures of necromass obtained from these cultures will then be looked for within soils.

Project Details

Start Date
2018-02-06
End Date
2018-09-30
Status
Closed

Team

Principal Investigator

Stephen Callister
Institution
Pacific Northwest National Laboratory

Team Members

Kirsten Hofmockel
Institution
Pacific Northwest National Laboratory