Elucidating synergistic plant-bacteria metabolic pathways for alleviating iron deficiency
EMSL Project ID
50160
Abstract
Nutrient mobilization from soil minerals is critical for plant growth, particularly in marginal lands with high pH soils or low phosphate availability. One way that rhizospheric bacteria enhance plant growth is by converting root exudates such as sugars and amino acids into organic acids and chelating molecules that enhance mineral dissolution and improve the availability of nutrients such as phosphorous and iron. The goal of the proposed project is to determine the effect of iron availability on metabolite exchange between Brachypodium distachyon and the rhizosphere bacteria Pseudomonas fluorescens and Bacillus subtillis. When trace metal availability is low, Brachypodium alters the composition of exudates to increase production of metabolites and organic acids that mediate metal uptake. In this project, we propose to address the following hypotheses:
(1) Growth under iron deficient versus iron replete conditions will reduce Brachypodium biomass and result in increased root exudation and increase bacterial colonization near the root tip.
(2) Siderophore and organic acid production by rhizosphere bacteria under iron deficient conditions will increase Brachypodium growth relative to sterile controls.
Project Details
Start Date
2018-03-22
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Rene M. Boiteau, Rosalie K. Chu, David W. Hoyt, Dehong Hu, Janet K. Jansson, Christer Jansson, Lye Meng Markillie, Hugh D. Mitchell, Ljiljana Pasa-Tolic. 2021. "Metabolic Interactions between
Brachypodium
and Pseudomonas fluorescens under Controlled Iron-Limited Conditions." mSystems 6 (1) 10.1128/mSystems.00580-20