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MS imaging of cell wall N- and O-linked glycans in interaction between roots and soil-borne microbes


EMSL Project ID
50168

Abstract

We propose to address a critical barrier in deciphering rhizosphere communication, specifically that of the role of glycans (a modifiable moiety of glycoproteins) in these processes. The first aim of the proposal is to develop a method to localize glycan structures along a root tissue, in effort to measure their spatiotemporal changes as it is related to specific soil microbiome interactions, the plant genotype, and environmental perturbations. To address this aim we will utilize mass spectrometry imaging (MSI) tools, namely the 15T-MALDI-FTICR-MS. The second aim is to relate alterations in glycan structure with physiological outcomes. Specifically, we will explore the modifications to these structures resulting from changes in the distribution of inter- and intracellular photosynthate and other small metabolites. This will be the first demonstration of imaging and molecular characterization of rhizosphere glycans, establishing EMSL on the leading-edge of this field.

Project Details

Start Date
2018-03-01
End Date
2018-04-16
Status
Closed

Team

Principal Investigator

Christopher Anderton
Institution
Environmental Molecular Sciences Laboratory

Team Members

Richard Drake
Institution
Medical University of South Carolina

Dusan Velickovic
Institution
Environmental Molecular Sciences Laboratory

Amir Ahkami
Institution
Environmental Molecular Sciences Laboratory