Structure and Chemical Mapping of Soil Organic Matter on Mineral Surfaces
EMSL Project ID
50184
Abstract
Secreted and decomposing soil organic matter (SOM) containing carbon, nitrogen, phosphorus, and metals form the basis of many bio/organic matter-mineral interactions in soils. These compounds are (bio)released by plants and microbes for many reasons – signal and nutrient exchange, interspecies biochemical warfare, disposal of metabolic waste products, and nutrient mobilization. The fate of these compounds, whether reassimilated by the same organism, or others, or sequestered and preserved on mineral surfaces (pristine or secondary) defines a critical component of the soil carbon and nutrient cycles and mineral dynamics that warrants further exploration. Innovative approaches are needed to better predict the affinity of SOM components to various solid phases within soils based on their molecular structure and functional groups. To address the capability gap of determining quantitatively where organic carbon partitions in a complex soil (e.g., mineral surfaces or particulate organic phases [POM]), we propose to use a suite of EMSL resources (experimental and modeling) to “develop isotopically-labeled chemical imaging and correlative spatial mapping techniques coupled with bulk and surface spectroscopic, microscopic, and modeling methods to gain insights into siderophore (e.g.,13C-Pyoverdine treated with 57Fe) – soil interactions in a natural model soil (Prosser, WA)” . The research objectives of this seed proposal are (a) to gain a comprehensive understanding of the mineralogy of Prosser soil, b) to utilize 13C and 57Fe isotopic labeling and chemical imaging and mapping methods to identify distribution/partitioning of pyoverdine on specific minerals present in soil, and c) to integrate this knowledge with modeling to gain a more holistic understanding of soil/microbial/mineral interactions.
Project Details
Start Date
2018-03-22
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Rene M. Boiteau, John B. Cliff, Alice Dohnalkova, Mark H. Engelhard, Kirsten S. Hofmockel, Libor Kovarik, Ravi Kukkadapu, James J. Moran, Daniel E. Perea, Chuck R. Smallwood, Tamas Varga, Thomas Wietsma, Mark G. Wirth. 2020. "Calcareous organic matter coatings sequester siderophores in alkaline soils." Science of The Total Environment 724:138250. 10.1016/j.scitotenv.2020.138250