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Mechanistic Study of Sulfur Oxidizing Bacteria Using a Combined Experimental and Theoretical Approach: The Role of the SoxB Protein


EMSL Project ID
50202

Abstract

The goal of this proposal is to characterize the molecular mechanisms associated to the a Mn2(II,II) core of the SoxB protein. This protein plays a central role in the sulfur oxidizing catalytic process used by bacteria to perform aerobic respiration or CO2 fixation. This will be achieved by using the integrated capability of EMSL to perform leading-edge cryogenic NMR and EPR spectroscopy measurements in combination with state-of-the-art correlated electronic structure calculations. To date, the mechanism for the action of the SoxB protein remains elusive. The proposed capability has the power to resolve the electronic structure of the Mn2(II,II) core of this protein and the mechanism for the action of this protein in the sulfur oxidizing catalytic process. The findings of this study will greatly impact our understanding of the elemental cycle of sulfur, but also the design of new bio-technologies related to sulfur-decontamination.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2018-10-01
End Date
2021-03-31
Status
Closed

Team

Principal Investigator

Ping Yang
Institution
Los Alamos National Laboratory

Co-Investigator(s)

Enrique Batista
Institution
Los Alamos National Laboratory

Team Members

Qing Guo
Institution
Michigan Technological University

Shanshan Wu
Institution
University of Illinois at Chicago

Bo Li
Institution
Los Alamos National Laboratory

Chang Liu
Institution
Los Alamos National Laboratory

David Gonzalez Alcantara
Institution
University of Puerto Rico at Rio Piedras

Anthony Schlimgen
Institution
Los Alamos National Laboratory

Biswas Rijal
Institution
Los Alamos National Laboratory

Stosh Kozimor
Institution
Los Alamos National Laboratory

Julie Jung
Institution
Los Alamos National Laboratory

Amanda Morgenstern
Institution
Los Alamos National Laboratory

Nestor Aguirre Castiblanco
Institution
Los Alamos National Laboratory

Gaoxue Wang
Institution
Los Alamos National Laboratory

Rebecca Carlson
Institution
Los Alamos National Laboratory

Morgan Kelley
Institution
Los Alamos National Laboratory

Jing Su
Institution
Los Alamos National Laboratory

Related Publications

Lehman-Andino I., J. Su, K.E. Papathanasiou, T.M. Eaton, J. Jian, D. Dan, and T.E. Albrecht-Schmitt, et al. 2019. "Soft-donor Dipicolinamide Derivatives for Selective Actinide(III)/Lanthanide(III) Separation: the Role of S- vs. O-donor Sites." ChemComm 55. doi:10.1039/c8cc07683a
Rice N.T., J. Su, T.P. Gompa, D.R. Russo, J. Telser, L. Palatinus, and J. Bacsa, et al. 2019. "Homoleptic Imidophosphorane Stabilization of Tetravalent Cerium." Inorganic Chemistry 58. doi:10.1021/acs.inorgchem.9b00368
Wang G., E.R. Batista, and P. Yang. 2018. "Ligand Induced Shape Transformation of Thorium Dioxide Nanocrystals." Physical Chemistry Chemical Physics. PCCP 20, no. 26:17563-17573. doi:10.1039/c8cp00240a
Wang G., P. Yang, N.A. Moody, and E.R. Batista. 2018. "Overcoming the Quantum Efficiency-lifetime Tradeoff of Photocathodes by Coating with Atomically Thin Two-dimensional Nanomaterials." npj 2D Materials and Applications. doi:10.1038/s41699-018-0062-6