Joining Systems Biology and Bioimaging on Same Cells
EMSL Project ID
50432
Abstract
We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote Ostreococcus tauri will help ensure a positive research outcome by providing a more compact template for probing the genotype/phenotype linkages with fewer redundant pathways. The need for combined approaches to generate a holistic understanding of biological complexity at the single- to few-cell level is reiterated in multiple recent DOE reports including the 2017 DOE Grand Challenges for Biological and Environmental Research: Progress and Future Vision report, the 2017 Technologies for Characterizing Molecular and Cellular Systems report and the 2014 BER Molecular Science Challenges report. To achieve our goals, we will pursue the following specific aims:
Aim 1: Perform dynamic imaging and single time-point omics on the same exact cells using an integrated workflow of separate instruments.
Aim 2: Develop a hybrid instrument for all-in-one on-chip cell loading, imaging, harvesting and nanoPOTS analysis to yield time-resolved imaging and omics of the same exact cells.
Project Details
Start Date
2018-10-19
End Date
2020-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Novikova I.V., N. Sharma, T.H. Moser, R.L. Sontag, Y. Liu, M. Collazo, and D.D. Cascio, et al. 2018. "Protein Structural Biology Using Cell-Free Platform from Wheat Germ." Advanced Structural and Chemical Imaging 4. PNNL-SA-136527. doi:10.1186/s40679-018-0062-9