Stable Isotope Probing Informatics Infrastructure for High Throughput Analyses
EMSL Project ID
50441
Abstract
The ability to identify, quantify and trace organic compounds through a soil ecosystem is essential for characterization of the molecular mechanisms that regulate biomolecule interactions with soil minerals. By tracking the incorporation of heavy isotopes into biomolecules, stable isotope probing (SIP) can offer a non-invasive technique for elucidating key molecules through space and time. Thorough mapping of these mechanisms requires measurements of multiple types of biomolecules (e.g., proteins, metabolites, lipids, etc.) utilizing a variety of analytical tools. EMSL houses the majority of the instrumentation needed to enable SIP of these molecules but currently lacks the informatics tools needed to reliably and quantitatively unlock this information from suitable mass spectra in a statistically relevant manner. We are proposing to create the informatics infrastructure necessary to build an automated, stable, statically robust and integrated stable isotope label based experimental platform for facile integration of a variety of analytical methods. These algorithms represent a critical piece of infrastructure upon which we will build EMSL's integrated SIP research campaigns. Leveraging the work from FY18 which focused on the development of an R based code for the analysis of proteomics data, we will validate the existing code with protein data, and then apply similar approaches to targeted and untargeted mass spectrometry based metabolomics data.
Project Details
Start Date
2018-10-11
End Date
2020-12-31
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members