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Prediction of soil microbiome phenotypic responses to changing moisture conditions


EMSL Project ID
50583

Abstract

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the molecular and ecological interactions occurring among natural microbial consortia. We propose a set of integrated experiments that together address the functional relationships of decomposing microbial communities and their response to changing moisture. We posit that moisture affects the physiology and interactions within and between consortia, resulting in predictable phenotypes. By defining phenotypes at the reduced complexity scale, we will be able to model and ultimately predict the soil microbiome's phenotypic response to changing moisture conditions, and to define the soil metaphenome as the product of community genomes (metagenomes) and environmental resources that are available.

Project Details

Start Date
2018-10-01
End Date
2019-09-30
Status
Closed

Team

Principal Investigator

Jason McDermott
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

Kirsten Hofmockel
Institution
Pacific Northwest National Laboratory

Team Members

Leslie Shor
Institution
University of Connecticut

Yi-Syuan Guo
Institution
Environmental Molecular Sciences Laboratory

Monee McGrady
Institution
Pacific Northwest National Laboratory

Katherine Schultz
Institution
Pacific Northwest National Laboratory

Glade Dlott
Institution
Stanford University

Ruonan Wu
Institution
Pacific Northwest National Laboratory

Arunima Bhattacharjee
Institution
Environmental Molecular Sciences Laboratory

Colin Brislawn
Institution
Pacific Northwest National Laboratory

Jamie Nunez
Institution
Pacific Northwest National Laboratory

Sean Colby
Institution
Pacific Northwest National Laboratory

Hyun-Seob Song
Institution
University of Nebraska - Lincoln

Joseph Brown
Institution
University of Utah

William Nelson
Institution
Pacific Northwest National Laboratory

Christopher Anderton
Institution
Environmental Molecular Sciences Laboratory

Janet Jansson
Institution
Pacific Northwest National Laboratory

Aaron Wright
Institution
Pacific Northwest National Laboratory

Ryan Renslow
Institution
Pacific Northwest National Laboratory

Thomas Metz
Institution
Pacific Northwest National Laboratory

Sriram Krishnamoorthy
Institution
Pacific Northwest National Laboratory

Related Publications

Bhattacharjee A., D. Velickovic, T.W. Wietsma, S.L. Bell, J.K. Jansson, K.S. Hofmockel, and C.R. Anderton. 2020. "Visualizing Microbial Community Dynamics Via a Controllable Soil Environment." mSystems 5, no. 1:Article No. e00645-19. PNNL-SA-147223. doi:10.1128/mSystems.00645-19