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Yarrowia lipolytica for biotechnology and synthetic biology


EMSL Project ID
50597

Abstract

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics/metabolomics to establish baseline cell biological and metabolite data on DNA agility in eukaryotic organisms, and applications of synthetic biology already in process. We will use gain and loss of innocuous phenotypes to track DNA moving through an environment It is important to establish genetic characteristics and conditions that contribute to environmental DNA uptake for an understanding of the consequences of organism release, but this information also have applications in NIH, agricultural, and defense subject areas. Carryover projects underway include study of a red-colored mutant, production of itaconic acid and associated implications for compartmentalization of cellular processes, and NADPH production and use from different enzymes and under different conditions. These investigations contribute to cell biology and contextualize bio-product production for engineering and modeling of improved strains.

Project Details

Start Date
2018-10-23
End Date
2019-09-30
Status
Closed

Team

Principal Investigator

Erin Bredeweg
Institution
Environmental Molecular Sciences Laboratory

Team Members

Rajendra Joshi
Institution
Pacific Northwest National Laboratory

Nathalie Munoz Munoz
Institution
Environmental Molecular Sciences Laboratory

Sean Colby
Institution
Pacific Northwest National Laboratory

Jeremy Zucker
Institution
Pacific Northwest National Laboratory

Dennis Thomas
Institution
Pacific Northwest National Laboratory

Neeraj Kumar
Institution
Pacific Northwest National Laboratory

Kyle Pomraning
Institution
Pacific Northwest National Laboratory

Eric Merkley
Institution
Pacific Northwest National Laboratory

Young-Mo Kim
Institution
Pacific Northwest National Laboratory

William Chrisler
Institution
Pacific Northwest National Laboratory

Scott Baker
Institution
Environmental Molecular Sciences Laboratory

Dehong Hu
Institution
Environmental Molecular Sciences Laboratory