Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.
EMSL Project ID
50629
Abstract
Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized tissues such as bone, cementum, dentin and enamel all consist of calcium phosphate, but have specialized functions as a result of the conditions during formation. Biomineralization proteins are an essential contributor to the resulting materials properties, as they orchestrate the nucleation, growth, and organization of biominerals. Understanding the fundamental mechanisms responsible for this orchestration is important because this knowledge will guide the design of advanced materials in general and new repair or regeneration strategies for biominerals.Project Details
Start Date
2018-11-07
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Jayasinha Arachchige R.M., S.D. Burton, J. Lu, B. Ginovska, L.K. Harding, M.E. Taylor, and J. Tao, et al. 2018. "Solid-State NMR Identification of Intermolecular Interactions in Amelogenin Bound to Hydroxyapatite." Biophysical Journal 115, no. 9:1666-1672. PNNL-SA-131727. doi:10.1016/j.bpj.2018.08.027
Tao J., Y. Shin, R.M. Jayasinha Arachchige, G.W. Buchko, S.D. Burton, A. Dohnalkova, and Z. Wang, et al. 2019. "The energetic basis for hydroxyapatite mineralization by amelogenin variants provides insights into the origin of amelogenesis imperfecta." Proceedings of the National Academy of Sciences of the United States of America 116, no. 28:13867-13872. PNNL-SA-143391. doi:10.1073/pnas.1815654116