Native Mass Spectrometry Enabled Electron Microscopy for Visual Proteomics
EMSL Project ID
50700
Abstract
Develop a soft-landing capability for native protein complexes coupled with electron microscopy (EM) to enable visual proteomics. Sample heterogeneity greatly limits the resolution of 3D structures and poses a severe bottleneck to processing image data generated by EM. To overcome this challenge, we will deposit mass selected ions of native protein complexes on graphene EM grids using recently developed native mass spectrometry (MS) capabilities at the EMSL. This capability will provide a more homogeneous population of particles on an atomically thin substrate yielding higher resolution and contrast, shorter data processing times, and the ability to characterized smaller protein complexes.
Project Details
Start Date
2019-01-21
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members