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Nanoliter-scale Autosampler for Seamless Integration of NanoPOTS Platform with LC-MS

EMSL Project ID
50931
Award DOI
https://dx.doi.org/10.46936/staf.proj.2019.50931/60006612

Abstract

 We propose to develop a high throughput, automated, and ultrasensitive single cell proteomic platform by seamlessly integrating our nanodroplet Processing in One-pot for Trace Sample technique with ultrasensitive liquid chromatography mass spectrometry. To accomplish our goal, the following Specific Aims will be pursued:
Aims 1: We will develop a new microfluidic sampling probe to directly introduce nanodroplets with volumes < 200 nL into high pressure valves.
Aims 2: We will develop an integrated nanoliter autosampler with analysis throughput of >50 samples per day.
Aims 3: We will demonstrate the utility of the new platform in proteomics-based typing of plant cells isolated from Brachypodium.

Project Details

Start Date
2019-05-13
End Date
2019-09-30
Status
Closed
Released Data Link
https://search.emsl.pnnl.gov/?project_id_search=50931

Team

Principal Investigator

Ying Zhu
Institution
Environmental Molecular Sciences Laboratory

Team Members

Andrey Liyu
Institution
Environmental Molecular Sciences Laboratory

Daniel Orton
Institution
Pacific Northwest National Laboratory

Ronald Moore
Institution
Pacific Northwest National Laboratory

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