Nanoliter-scale Autosampler for Seamless Integration of NanoPOTS Platform with LC-MS
EMSL Project ID
50931
Abstract
We propose to develop a high throughput, automated, and ultrasensitive single cell proteomic platform by seamlessly integrating our nanodroplet Processing in One-pot for Trace Sample technique with ultrasensitive liquid chromatography mass spectrometry. To accomplish our goal, the following Specific Aims will be pursued:Aims 1: We will develop a new microfluidic sampling probe to directly introduce nanodroplets with volumes < 200 nL into high pressure valves.
Aims 2: We will develop an integrated nanoliter autosampler with analysis throughput of >50 samples per day.
Aims 3: We will demonstrate the utility of the new platform in proteomics-based typing of plant cells isolated from Brachypodium.
Project Details
Start Date
2019-05-13
End Date
2019-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members