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Targeted spatial metabolomics: an automated approach to identifying and analyzing single cells in situ


EMSL Project ID
51149

Abstract

Tissue-scale or cell-population-level analysis (i.e., ‘bulk analysis’) yields averaged abundance data that can mask critical information about the state of individual cells. Through a recently ended DOE-BER Bioimaging grant, we demonstrated the ability to attain single cell metabolomics data using a fiber-based laser ablation electrospray ionization (f-LAESI) mass spectrometry (MS) approach. Here, we propose to move beyond these proof-of-concept experiments to automate this method in an effort to make this unique capability available to the EMSL User Community (Fig. 1). We will also demonstrate the universality of this f-LAESI setup by coupling it to both ion-mobility-based and Fourier transform-based mass spectrometers. This work will expand on the conventional LAESI source currently being constructed and tested under a funded PNNL Open Call LDRD project.

Project Details

Start Date
2019-10-01
End Date
2021-03-31
Status
Closed

Team

Principal Investigator

Christopher Anderton
Institution
Environmental Molecular Sciences Laboratory

Team Members

Michael Taylor
Institution
Private Industry (Roop)

Jessica Lukowski
Institution
Environmental Molecular Sciences Laboratory

Amir Ahkami
Institution
Environmental Molecular Sciences Laboratory

Akos Vertes
Institution
The George Washington University

Andrey Liyu
Institution
Environmental Molecular Sciences Laboratory