Targeted spatial metabolomics: an automated approach to identifying and analyzing single cells in situ
EMSL Project ID
51149
Abstract
Tissue-scale or cell-population-level analysis (i.e., ‘bulk analysis’) yields averaged abundance data that can mask critical information about the state of individual cells. Through a recently ended DOE-BER Bioimaging grant, we demonstrated the ability to attain single cell metabolomics data using a fiber-based laser ablation electrospray ionization (f-LAESI) mass spectrometry (MS) approach. Here, we propose to move beyond these proof-of-concept experiments to automate this method in an effort to make this unique capability available to the EMSL User Community (Fig. 1). We will also demonstrate the universality of this f-LAESI setup by coupling it to both ion-mobility-based and Fourier transform-based mass spectrometers. This work will expand on the conventional LAESI source currently being constructed and tested under a funded PNNL Open Call LDRD project.
Project Details
Start Date
2019-10-01
End Date
2021-03-31
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members