Structures of secondary membrane transporters
EMSL Project ID
51179
Abstract
We aim to determine the structures of several nutrient and drug transporter proteins. These membrane transporters play major physiological or pharmacological roles, and high resolution structural information is needed to understand their molecular mechanisms. We will pay special attention to sample preparation, by employing various protection media for grid freezing, including detergents, nanodisc and amphipol. To increase the mass of the proteins for more accurate particle alignment, we have generated conformational specific Fab Fragments of all the targeted transporter proteins. In the previous cycle of microscope sessions at PNCC, we were able to obtain several maps at ~3.5 Å resolution, and we now have grids ready and therefore expect even better results in the next cycle.Project Details
Start Date
2019-11-10
End Date
2020-09-10
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Sauer et al. Structural basis for the reaction cycle of DASS dicarboxylate transporters eLife 2020 Sep 1;9:e61350. doi: 10.7554/eLife.61350.