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Structures of secondary membrane transporters


EMSL Project ID
51179

Abstract

We aim to determine the structures of several nutrient and drug transporter proteins. These membrane transporters play major physiological or pharmacological roles, and high resolution structural information is needed to understand their molecular mechanisms. We will pay special attention to sample preparation, by employing various protection media for grid freezing, including detergents, nanodisc and amphipol. To increase the mass of the proteins for more accurate particle alignment, we have generated conformational specific Fab Fragments of all the targeted transporter proteins. In the previous cycle of microscope sessions at PNCC, we were able to obtain several maps at ~3.5 Å resolution, and we now have grids ready and therefore expect even better results in the next cycle.

Project Details

Start Date
2019-11-10
End Date
2020-09-10
Status
Closed

Team

Principal Investigator

Da-Neng Wang
Institution
New York University School of Medicine

Team Members

Douglas Brawley
Institution
New York University School of Medicine

Theo Humphreys
Institution
Oregon Health & Science University

David Sauer
Institution
New York University School of Medicine

Related Publications

Sauer et al. Structural basis for the reaction cycle of DASS dicarboxylate transporters eLife 2020 Sep 1;9:e61350. doi: 10.7554/eLife.61350.