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Structural studies on chromatin assembly complexes


EMSL Project ID
51216

Abstract

Nucleosome is the fundamental unit of DNA high-order structure called chromatin. Nucleosome is a complicated structure composed of four different histones and DNA. The assembly of nucleosome is highly regulated upon cellular processes. Several protein complexes are involved in assembling nucleosome. Among these, CAF-1 directly deposits histone H3-H4 followed by H2A-H2B deposition by FACT. In addition, our group recently showed that Abo1 also deposits histone H3-H4 in an ATP-dependent manner. Despite of the importance of nucleosome assembly, little is known about the mechanism behind nucleosome assembly. Therefore, in this proposal, we aim 1) to determine the cryo-EM structure of Abo1 bound with histone H3-H4, and 2) to obtain the cryo-EM structure of CAF-1 bound with histone H3-H4. The structures of CAF-1 and Abo1 complexes with histone H3-H4 will provide mechanistic understanding on how these complexes recognizes their substrate and load them on DNA to form nucleosome.

Project Details

Start Date
2019-12-20
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Ji-Joon Song
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Team Members

Juwon Jang
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Gijeong Kim
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Carol Cho
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Theo Humphreys
Institution
Oregon Health & Science University