Structural studies on chromatin assembly complexes
EMSL Project ID
51216
Abstract
Nucleosome is the fundamental unit of DNA high-order structure called chromatin. Nucleosome is a complicated structure composed of four different histones and DNA. The assembly of nucleosome is highly regulated upon cellular processes. Several protein complexes are involved in assembling nucleosome. Among these, CAF-1 directly deposits histone H3-H4 followed by H2A-H2B deposition by FACT. In addition, our group recently showed that Abo1 also deposits histone H3-H4 in an ATP-dependent manner. Despite of the importance of nucleosome assembly, little is known about the mechanism behind nucleosome assembly. Therefore, in this proposal, we aim 1) to determine the cryo-EM structure of Abo1 bound with histone H3-H4, and 2) to obtain the cryo-EM structure of CAF-1 bound with histone H3-H4. The structures of CAF-1 and Abo1 complexes with histone H3-H4 will provide mechanistic understanding on how these complexes recognizes their substrate and load them on DNA to form nucleosome.
Project Details
Start Date
2019-12-20
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members