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Structural studies of the targeting factor Get3


EMSL Project ID
51234

Abstract

Tail-anchored (TA) proteins, accounting for approximately 2% of the genome, are a class of proteins with various functions but all with a single transmembrane domain near their C-terminus. Due to the location of their only transmembrane domains these proteins cannot access the traditional protein targeting pathways and must be trafficked post-translationally. The first identified and most studied pathway for targeting TA proteins to the ER membrane is the Guided Entry of TA protein (GET) pathway. The central targeting factor of the GET pathway is the ATPase Get3. While many eukaryotic structures of Get3 have been solved, one critical structure missing is the post-hydrolysis state of Get3 still bound to TA protein. We aim to solve this structure using recently identified homolog of Get3 in the single cell eukaryote Giardia intestinalis using cryo-EM.

Project Details

Start Date
2019-12-20
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

William Clemons
Institution
California Institute of Technology

Team Members

Michelle Fry
Institution
California Institute of Technology

Theo Humphreys
Institution
Oregon Health & Science University