Structural studies of the targeting factor Get3
EMSL Project ID
51234
Abstract
Tail-anchored (TA) proteins, accounting for approximately 2% of the genome, are a class of proteins with various functions but all with a single transmembrane domain near their C-terminus. Due to the location of their only transmembrane domains these proteins cannot access the traditional protein targeting pathways and must be trafficked post-translationally. The first identified and most studied pathway for targeting TA proteins to the ER membrane is the Guided Entry of TA protein (GET) pathway. The central targeting factor of the GET pathway is the ATPase Get3. While many eukaryotic structures of Get3 have been solved, one critical structure missing is the post-hydrolysis state of Get3 still bound to TA protein. We aim to solve this structure using recently identified homolog of Get3 in the single cell eukaryote Giardia intestinalis using cryo-EM.
Project Details
Start Date
2019-12-20
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members