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Structural studies of chromatin complexes involved in human diseases


EMSL Project ID
51260

Abstract

The nucleosome is a fundamental unit of chromatin and the target of a host of gene regulatory chromatin enzymes and proteins. It consists of DNA wrapped around a protein core, composed of histones. Despite many efforts, structural and mechanistic understanding of how important chromatin modifiers bind and alter their nucleosome substrates is often lacking. This information would allow us a glimpse into the mechanisms of many factors important in human diseases. Our laboratory has successfully reconstituted many of these enzymes bound to their nucleosome substrate. Our preliminary cryo-EM results indicate the presence of flexibility within our targets chromatin complexes. We seek to access the PNCC facility to acquire a large number of images and subsequently a large number of particles which will be crucial to obtain good quality maps of our chromatin complexes

Project Details

Start Date
2020-01-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Song Tan
Institution
Pennsylvania State University

Team Members

Jose Espinola Lopez
Institution
Pennsylvania State University

Priit Eek
Institution
Pennsylvania State University

Othman Rechiche
Institution
Pennsylvania State University

Jean-Paul Armache
Institution
Pennsylvania State University

Theo Humphreys
Institution
Oregon Health & Science University

Irina El Khoury
Institution
Environmental Molecular Sciences Laboratory