Structural studies of chromatin complexes involved in human diseases
EMSL Project ID
51260
Abstract
The nucleosome is a fundamental unit of chromatin and the target of a host of gene regulatory chromatin enzymes and proteins. It consists of DNA wrapped around a protein core, composed of histones. Despite many efforts, structural and mechanistic understanding of how important chromatin modifiers bind and alter their nucleosome substrates is often lacking. This information would allow us a glimpse into the mechanisms of many factors important in human diseases. Our laboratory has successfully reconstituted many of these enzymes bound to their nucleosome substrate. Our preliminary cryo-EM results indicate the presence of flexibility within our targets chromatin complexes. We seek to access the PNCC facility to acquire a large number of images and subsequently a large number of particles which will be crucial to obtain good quality maps of our chromatin complexes
Project Details
Start Date
2020-01-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members