Analysis of two dimensional crystals of membrane-bound HIV-1 proteins
EMSL Project ID
51288
Abstract
Despite advances in AIDS diagnosis and treatment, the continuing AIDS epidemic demands continuing efforts to understand all aspects of HIV replication and to develop new methods for its inhibition. One target for viral interference is the interaction between HIV-1 MA and Env proteins. At the present time, models for how MA and Env interact are based largely on our lab's negative stain electron microscopy (EM) analyses of how MA proteins organize on membranes. Unfortunately, the resolutions achieved in these studies were insufficient to distinguish how interactions might be affected by MA mutations that block incorporation of Env proteins into virions, and of mutations that suppress Env incorporation defects. However, we recently have modified our protocols to extend our analyses via cryo-EM. The results we obtain will help elucidate how MA and Env proteins interact, and will foster the development of novel approaches to interfere with HIV-1 replication.
Project Details
Start Date
2020-03-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members