The cryoEM structures of the MMOH-MMOR complex
EMSL Project ID
51325
Abstract
Methane to methanol conversion is critical for environmental health, reducing greenhouse gas, and utilizing the natural gas as an alternative energy source. Methanotrophic bacteria utilize the methane (CH4) as a sole source of carbon and energy by oxidizing and breaking the highly stable C-H bond. This can be achieved via the enzyme complex called methane monooxygenase (MMO). Soluble methane monooxygenase (sMMO) is a multi-component protein complex comprising a 245-kDa (alpha beta gamma)2 hydroxylase MMOH, a 16-kDa regulatory protein MMOB, a 38-kDa electron-transfer reductase MMOR, and 12-kDa potential MMO inhibitory subunit (MMOD). Since the maximum catalytic activity of sMMO is only achieved via the interplay of MMOH with three auxiliary subunits, structural elucidation is a key to understand its chemistry and to develop bio-mimetic catalysts. In this project, we would like to illustrate how auxiliary subunits interact with MMOH using single-particle cryo-EM.
Project Details
Start Date
2020-05-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members