Structural characterization of BlaR1: A key regulator of beta-lactam resistance in Staphylococcus aureus
EMSL Project ID
51337
Abstract
Our vision is to develop and use structural biology methods to characterize membrane-associated protein assemblies relevant to antibiotic resistance and bacterial pathogenicity. We are particularly interested in understanding how antibiotic resistance is regulated in the multidrug resistant pathogen, methicillin resistant Staphylococcus aureus (MRSA). We aim to structurally characterize BlaR1, a key integral membrane signaling protein facilitating ?-lactam antibiotic resistance in MRSA. BlaR1 is activated when it is acylated by ?-lactam antibiotics, triggering the expression of key genes needed for antibiotic resistance in MRSA. We propose to use cryo-electron microscopy to determine the structures of BlaR1 in various states of activation to illuminate its mechanism of action and to aid in the design of novel antibiotics to control and prevent drug resistant bacterial infections.
Project Details
Start Date
2020-05-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members