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Elucidating Native Structures of Enveloped Virus Glycoproteins using Cryo-EM and Tomography


EMSL Project ID
51345

Abstract

We seek to understand the entry mechanisms of enveloped viruses for three viral families having broad health impact worldwide: coronaviruses such as SARS CoV 2, retroviruses such as HIV 1, and orthomyxoviruses such as influenza A H1N1. The project aims to determine sub-nanometer structures of envelope glycoprotein on native non-infectious pseudovirions. This advance will allow investigating the nature of the interaction between viral glycoproteins and human host receptors, and studying the vulnerability of these interactions to antibody-mediated neutralization. The aims of this project are related to our overall program to improve drug treatments and for rational vaccine design.

Project Details

Start Date
2020-05-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Sriram Subramaniam
Institution
The University of British Columbia

Team Members

Oleg Kuybeda
Institution
The University of British Columbia

Jean-Philippe Demers
Institution
The University of British Columbia

Alison Berezuk
Institution
The University of British Columbia

Xing Zhu
Institution
The University of British Columbia

Sagar Chittori
Institution
The University of British Columbia

Theo Humphreys
Institution
Oregon Health & Science University