Skip to main content

Understanding the structural basis of NLR proteins in regulating MHC gene expression


EMSL Project ID
51487

Abstract

Class II transcription activator (CIITA) is immunologically critical for T cell maturation as it regulates the Major Histocompatibility Complex class II (MHC-II) gene expression. Unlike the other transcription factors, CIITA is recruited by the pre-formed RFX5/CREB/NF-Y/DNA complex to the promoter region rather than directly interact with DNA. As a master regulator of MHC class II gene expression, CIITA is targeted by various cytokines, viral factors and cancer related molecules. However, the underlying mechanism of how CIITA regulates gene expression remains to be addressed. Here, we propose to use Arctica to examine the cryoEM sample of recombinant CIITA and to collect a set of cryo-EM data for 3D reconstruction, which will provide preliminary data for R01 application and help us to further improve the sample preparation.

Project Details

Start Date
2020-06-15
End Date
2020-09-15
Status
Closed

Team

Principal Investigator

Liman Zhang
Institution
Oregon Health & Science University

Team Members

Bhaskar Paidimuddala
Institution
Oregon Health & Science University

Jianhao Cao
Institution
Oregon Health & Science University