Integrative analysis of multi-omic data across NEON eco-climatic regions
EMSL Project ID
51541
Abstract
Our climate crisis, resulting from changes in interacting climate variables (temperature, rainfall, atmospheric chemistry) over the last century, has impacted all ecosystems on the surface of the Earth. With modern DNA sequencing techniques it is now possible to simultaneously sample thousands of different species, providing a window into the diverse soil organismal community and their ecological traits. Similarly with high resolution mass spectroscopy (e.g FT-ICR MS) it is possible to detect thousands of organic molecules leading to insights into changes in soil composition over time. This proposal emanates from members of two NSF LTER synthesis working groups "Ecological Metagenome-derived Reference Genomes and Traits (EMERGENT)" and "Soil Data Harmonization (SoDaH)" in response to the Focused Topic Area "Utilize soils from the NEON Biorepository and EMSL/JGI capabilities to conduct continental scale ecosystem research". In keeping with the focus of the call our project will "investigate the soil chemistry and soil microbial communities across NEON sites along climate/vegetation gradients".We propose to augment NEON data products with -omics approaches available through the DOE FICUS program (mini-metagenomes, metatranscriptomes, soil organic matter chemistry, lipidomics and metametabolomes). This data provides a foundation for exciting new frameworks that incorporate genomic sequence and soil organic matter data into mathematical models, and in this way approach the problem of scaling cell metabolism to ecosystem-level models.
Project Details
Project type
FICUS Research
Start Date
2020-10-01
End Date
2022-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)