Cryo-EM structural analysis of Glucose-6-phosphatase catalytic subunit, G6PC1
EMSL Project ID
51576
Abstract
The catalytic subunit of glucose-6-phosphatase, G6PC1, functions as a gatekeeper of glucose production in the liver by catalyzing the hydrolysis of glucose-6-phosphate. Dysregulation of G6PC1 gene expression contributes to elevated hepatic glucose production associated with diabetes, and more than 100 debilitating mutations lead to glycogen storage disease type 1a. There are no high-resolution structures of G6PC1, which would support a more complete understanding of its mechanistic biology. This proposal aims to obtain the first atomic-scale models of G6PC1 by collecting cryo-EM datasets on the PNCC Krios. We request a total of 72 hrs of microscope time. The ultimate goal of these efforts is to define the molecular architecture of G6PC1 in order to identify the relative position of disease-causing mutations and protein-protein interaction motifs. The results of this work will set the stage for a description of the structural basis of G6PC1 activity and the relationship to disease-associated mutations.
Project Details
Start Date
2020-09-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
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