Toward the visualization of eukaryotic transcription machineries by cryo-EM
EMSL Project ID
51582
Abstract
Eukaryotic genomes are modulated by different types of chromatin remodeling complexes and transcribed by multiple RNA polymerases. Of the three polymerases, Pol I-III, that produce RNA required for protein production, key structural information for the regulation of Pol II and Pol III has yet to be fully elucidated. While in vitro reconstitution of ISWI-dinucleosome complex is still underway, we are able to reconstitute in vitro pre-initiation complexes (PICs) for some of these polymerases. Our preliminary cryo-EM reconstruction of the human Mediator-bound Pol II PIC and Pol III initiation complex have both been solved to sub-nanometer resolution. However, the current resolution prevents accurate model building of these important models. High resolution information for these complexes is necessary to understand their regulation and how mis-regulation of these complexes leads to human disease.
Project Details
Start Date
2020-07-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Abdella, R., A. Talyzina, S. Chen, C. J. Inouye, R. Tjian, and Y. He. "Structure of the human Mediator-bound transcription preinitiation complex." Science (2021).Abdella, R., A. Talyzina, S. Chen, C. J. Inouye, R. Tjian, and Y. He. "Structure of the human Mediator-bound transcription preinitiation complex." Science (2021).