Next generation microfluidics platform for high throughput single cell proteomics
EMSL Project ID
51583
Abstract
The project aims to improve both sensitivity and throughput in single cell proteomics through the development of a microfluidic platform that leverages current technology. A hybrid picowell/nanowell chip will be designed and tested for single cell proteomics on a commercially available robotic system. The resulting platform will be demonstrated by profiling protein expressions from >1000 single plant cells.
Project Details
Start Date
2020-06-26
End Date
2020-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Joshua N. Adkins, Victor Aguilera-Vazquez, Joshua Cantlon-Bruce, Geremy C. Clair, Song Feng, Dehong Hu, Tao Liu, Lye Meng Markillie, Hardeep S. Mehta, Ronald J. Moore, Ljiljana Pasa-Tolic, Richard D. Smith, Ryan L. Sontag, Chia-Feng Tsai, Sarah M. Williams, Jongmin Woo, Ying Zhu. 2021. "High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip." Nature Communications 12 (1) 10.1038/s41467-021-26514-2