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Next generation microfluidics platform for high throughput single cell proteomics


EMSL Project ID
51583

Abstract

Abstract

The project aims to improve both sensitivity and throughput in single cell proteomics through the development of a microfluidic platform that leverages current technology. A hybrid picowell/nanowell chip will be designed and tested for single cell proteomics on a commercially available robotic system. The resulting platform will be demonstrated by profiling protein expressions from >1000 single plant cells.

Project Details

Start Date
2020-06-26
End Date
2020-09-30
Status
Closed

Team

Principal Investigator

Ying Zhu
Institution
Environmental Molecular Sciences Laboratory

Co-Investigator(s)

Mowei Zhou
Institution
Environmental Molecular Sciences Laboratory

Team Members

Vimal Kumar Balasubramanian
Institution
Environmental Molecular Sciences Laboratory

Sarah Williams
Institution
Environmental Molecular Sciences Laboratory

Amir Ahkami
Institution
Environmental Molecular Sciences Laboratory

William Chrisler
Institution
Pacific Northwest National Laboratory

Related Publications

Joshua N. Adkins, Victor Aguilera-Vazquez, Joshua Cantlon-Bruce, Geremy C. Clair, Song Feng, Dehong Hu, Tao Liu, Lye Meng Markillie, Hardeep S. Mehta, Ronald J. Moore, Ljiljana Pasa-Tolic, Richard D. Smith, Ryan L. Sontag, Chia-Feng Tsai, Sarah M. Williams, Jongmin Woo, Ying Zhu. 2021. "High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip." Nature Communications 12 (1) 10.1038/s41467-021-26514-2