Cryo-EM studies of the prothrombinase:prothrombin complex and the protein C anti-coagulant complex
EMSL Project ID
51604
Abstract
The objective of this study, funded by 2 active NIH grants, is to elucidate the structures of key proteins and their interacting partners of the prothrombinase:prothrombin complex and the protein C anti-coagulant complex. This study addresses two central interactions of the blood coagulation cascade in order to generate basic knowledge for translational opportunities to drive the treatment of pathologies that affect millions of people worldwide. We aim to use cryo-em to derive structural information for full-length prothrombin, protein C, factor Va, the prothrombin:prothrombinase complex and the protein C anti-coagulant complex. This project is currently limited by SLU lacking an onsite cryo-em facility and restricted access to the nearby Washington University, St Louis facility. Access to PNCC will greatly accelerate this project. In addition, the use of state-of-the-art instruments and especially, professional help and training in data collection techniques, such as use of the phase plate, will greatly improve data quality.
Project Details
Start Date
2020-08-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members