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Cryo-EM studies of the prothrombinase:prothrombin complex and the protein C anti-coagulant complex


EMSL Project ID
51604

Abstract

The objective of this study, funded by 2 active NIH grants, is to elucidate the structures of key proteins and their interacting partners of the prothrombinase:prothrombin complex and the protein C anti-coagulant complex. This study addresses two central interactions of the blood coagulation cascade in order to generate basic knowledge for translational opportunities to drive the treatment of pathologies that affect millions of people worldwide. We aim to use cryo-em to derive structural information for full-length prothrombin, protein C, factor Va, the prothrombin:prothrombinase complex and the protein C anti-coagulant complex. This project is currently limited by SLU lacking an onsite cryo-em facility and restricted access to the nearby Washington University, St Louis facility. Access to PNCC will greatly accelerate this project. In addition, the use of state-of-the-art instruments and especially, professional help and training in data collection techniques, such as use of the phase plate, will greatly improve data quality.

Project Details

Start Date
2020-08-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Enrico Di Cera
Institution
Saint Louis University

Team Members

Eliza Ruben
Institution
Saint Louis University

Irina El Khoury
Institution
Environmental Molecular Sciences Laboratory

Trevor Moser
Institution
Environmental Molecular Sciences Laboratory