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Structural investigation of multi-modular chromatin modifying complexes


EMSL Project ID
51616

Abstract

Post-translational modification of histone proteins is a key mechanism that eukaryotic cells use to regulate transcription. NuA4 and SAGA are two highly conserved histone aceyltransferase complexes that have served as key models for understanding fundamental principles governing histone modifications. Both NuA4 and SAGA complexes consists of multiple protein subunits that are organized into independent modules. However, how these modules coordinate with one another to regulate substrate selection and catalysis are not fully understood. This is due in part to technical challenges associated with structural characterization of these large MegaDalton-sized complexes which have sophisticated composition and exhibit inherent conformation flexibility. Having already established robust methods for isolating and vitrifying yeast NuA4 and SAGA, we are proposing to use cryo-EM to address important biological questions concerning the function and regulation of these histone modifying complexes.

Project Details

Start Date
2020-09-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Calvin Yip
Institution
The University of British Columbia

Team Members

Diana Vasyliuk
Institution
The University of British Columbia

Udit Dalwadi
Institution
The University of British Columbia