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Spatially resolved chemical proteomics for linking enzymatic activity to omics phenotyping in heterogeneous microbial communities


EMSL Project ID
60001

Abstract

We will connect chemical probes and nanoPOTS to rapidly identify microbial species and essential protein functions in structured microbial communities. We will use aggregation induced emission (AIE) probes to visualize hotspots of specific enzyme activities (N-acetyl-beta-D-glucosaminidase [NAG], phosphatase) in biofilms. Hotspots will be excised using laser capture microdissection (LCM) and processed by nanoPOTS. This spatially resolved chemical proteomics technology integrates functional analysis with protein identification in small, low protein samples. Successful outcome will significantly contribute to EMSL’s Cellular Signaling and Communications IRP by providing a means to identify sites of function and more rapidly characterize the enzymes responsible for these functions within complex (multi-species) structured samples.

Project Details

Start Date
2021-04-08
End Date
2022-06-30
Status
Closed

Team

Principal Investigator

Vivian Lin
Institution
Pacific Northwest National Laboratory

Team Members

Ying Zhu
Institution
Environmental Molecular Sciences Laboratory

Kirsten Hofmockel
Institution
Pacific Northwest National Laboratory

Natalie Sadler
Institution
Pacific Northwest National Laboratory

William Chrisler
Institution
Pacific Northwest National Laboratory