Spatially resolved chemical proteomics for linking enzymatic activity to omics phenotyping in heterogeneous microbial communities
EMSL Project ID
60001
Abstract
We will connect chemical probes and nanoPOTS to rapidly identify microbial species and essential protein functions in structured microbial communities. We will use aggregation induced emission (AIE) probes to visualize hotspots of specific enzyme activities (N-acetyl-beta-D-glucosaminidase [NAG], phosphatase) in biofilms. Hotspots will be excised using laser capture microdissection (LCM) and processed by nanoPOTS. This spatially resolved chemical proteomics technology integrates functional analysis with protein identification in small, low protein samples. Successful outcome will significantly contribute to EMSL’s Cellular Signaling and Communications IRP by providing a means to identify sites of function and more rapidly characterize the enzymes responsible for these functions within complex (multi-species) structured samples.
Project Details
Start Date
2021-04-08
End Date
2022-06-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members