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Deep UV laser ablation system for spatial single-cell proteomics


EMSL Project ID
60120

Abstract

Laser capture microdissection (LCM) is the key capability for spatially resolved omicss. However, commercially available LCM systems are designed for mammalian tissues and feature UV lasers (330 nm), which are inefficient to cut through plant tissues, primarily because of the presence of cell walls. We aim to overcome this significant technical obstacle by designing and constructing a deep UV (DUV, 193 nm) LCM system. We will couple the newly developed system with nanoPOTS technology to enable spatially resolved single cell proteomics and demonstrate its suitability with the study of actinorhizal plant symbiosis between red alder (Alnus rubra) and Frankia bacteria.

Project Details

Start Date
2021-10-01
End Date
N/A
Status
Active

Team

Principal Investigator

William Chrisler
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

Ying Zhu
Institution
Environmental Molecular Sciences Laboratory

Team Members

Liang Chen
Institution
Environmental Molecular Sciences Laboratory

Lisa Bramer
Institution
Pacific Northwest National Laboratory

Andrey Liyu
Institution
Environmental Molecular Sciences Laboratory

Kim Hixson
Institution
Pacific Northwest National Laboratory

Ljiljana Pasa-Tolic
Institution
Environmental Molecular Sciences Laboratory

Dehong Hu
Institution
Environmental Molecular Sciences Laboratory