Deep UV laser ablation system for spatial single-cell proteomics
EMSL Project ID
60120
Abstract
Laser capture microdissection (LCM) is the key capability for spatially resolved omicss. However, commercially available LCM systems are designed for mammalian tissues and feature UV lasers (330 nm), which are inefficient to cut through plant tissues, primarily because of the presence of cell walls. We aim to overcome this significant technical obstacle by designing and constructing a deep UV (DUV, 193 nm) LCM system. We will couple the newly developed system with nanoPOTS technology to enable spatially resolved single cell proteomics and demonstrate its suitability with the study of actinorhizal plant symbiosis between red alder (Alnus rubra) and Frankia bacteria.
Project Details
Start Date
2021-10-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members