Harnessing Biomineralization Potential of Metal-Oxidizing Bacteria for REE Synthesis
EMSL Project ID
60160
Abstract
Although redox reactions and enzymes participating in the extracellular oxidation of Fe and Mn are identified, little is known about the biomineralization mechanisms that facilitate formation secondary ferromanganese minerals that facilitate capture and concentration of REEs. There is also lack of understanding regarding molecular interactions between metals and biological molecules displayed on the surface of the cell that catalyze metal binding and nucleation reactions. As a steppingstone toward realizing the full potential of genetically encoded material synthesis, we propose to: (i) investigate the in vivo dynamics and putative mechanisms of REE biomineralization and sequestration by Fe/Mn oxidizing proteobacteria (e.g., S. lithotrophicus); (ii) apply a suite of omics, microscopic and spectroscopic techniques to characterize Fe/Mn/REE nanoparticle assembly at the abiotic-biotic interface (stalks); (iii) develop genetic tools to modulate biochemical activities and express synthetic scaffolds in Fe/Mn oxidizing zeta-proteobacteria (S. lithotrophicus) for selective REE binding; and (iv) establish working collaboration with Dr. Clara Chan (Univ Delaware) . The proposed work builds upon PNNL’s scientific strengths in microbial functional genomics, environmental microbiology and core capabilities in characterizing biominerals/biomineralization proteins using AFM, TEM, SEM and cryo-EM. It also provides a foundation for developing a productive research collaboration with Prof. Clara Chan (Univ. of Delaware), a leading expert in Mn/Fe oxidizing bacteria, ultimately positioning PNNL for anticipated funding opportunities from the Office of Biological & Environmental Research (BER) on the topic of Genetically Encoded Materials.
Project Details
Start Date
2021-07-20
End Date
2021-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members