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Nanoliter-scale Affinity Purification Mass Spectrometry for Small Numbers of Hair Cells


EMSL Project ID
60218

Abstract

The molecular mechanisms of mechanotransduction function of hair cells in auditory system and the vestibular system are largely undefined due to the scarcity of hair cells and the lack of sensitive measurement methods. The goal of this proposal is to overcome current fundamental technical challenges and develop a sensitive affinity purification with mass spectrometry (AP-MS) platform to discover protein interaction partners and establish protein-protein-interaction networks from small number of isolated hair cells.

Project Details

Start Date
2021-11-19
End Date
N/A
Status
Active

Team

Principal Investigator

Ying Zhu
Institution
Environmental Molecular Sciences Laboratory

Team Members

Liang Chen
Institution
Environmental Molecular Sciences Laboratory

Piliang Xiang
Institution
Environmental Molecular Sciences Laboratory

Lye Meng Markillie
Institution
Environmental Molecular Sciences Laboratory

William Chrisler
Institution
Pacific Northwest National Laboratory

Related Publications

Yumi Kwon, Paul D. Piehowski, Rui Zhao, Ryan L. Sontag, Ronald J. Moore, Kristin E. Burnum-Johnson, Richard D. Smith, Wei-Jun Qian, Ryan T. Kelly, Ying Zhu. 2022. "Hanging drop sample preparation improves sensitivity of spatial proteomics." Lab on a Chip 22 (15):2869-2877. https://doi.org/10.1039/d2lc00384h