Nanoliter-scale Affinity Purification Mass Spectrometry for Small Numbers of Hair Cells
EMSL Project ID
60218
Abstract
The molecular mechanisms of mechanotransduction function of hair cells in auditory system and the vestibular system are largely undefined due to the scarcity of hair cells and the lack of sensitive measurement methods. The goal of this proposal is to overcome current fundamental technical challenges and develop a sensitive affinity purification with mass spectrometry (AP-MS) platform to discover protein interaction partners and establish protein-protein-interaction networks from small number of isolated hair cells.
Project Details
Start Date
2021-11-19
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Yumi Kwon, Paul D. Piehowski, Rui Zhao, Ryan L. Sontag, Ronald J. Moore, Kristin E. Burnum-Johnson, Richard D. Smith, Wei-Jun Qian, Ryan T. Kelly, Ying Zhu. 2022. "Hanging drop sample preparation improves sensitivity of spatial proteomics." Lab on a Chip 22 (15):2869-2877. https://doi.org/10.1039/d2lc00384h